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Literature summary extracted from
- Nicotinamide nucleotide transhydrogenases (1976), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 13, 51-88.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | 2'-AMP | - |
Pseudomonas fluorescens |  |
| 1.6.1.1 | 2'-AMP | - |
Pseudomonas aeruginosa | |
| 1.6.1.1 | 2'-AMP | - |
Azotobacter vinelandii | |
| 1.6.1.1 | 2'-AMP | - |
Azotobacter agilis | |
| 1.6.1.1 | 2'-AMP | - |
Azotobacter chroococcum | |
| 1.6.1.1 | p-hydroxymercuribenzoate | activation, depending on presence of oxidized or reduced substrates | Pseudomonas fluorescens | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | 2'-AMP | - |
Chromatium sp. | |
| 1.6.1.1 | p-hydroxymercuribenzoate | - |
Azotobacter vinelandii | |
| 1.6.1.1 | p-hydroxymercuribenzoate | - |
Pseudomonas aeruginosa | |
| 1.6.1.1 | p-hydroxymercuribenzoate | dependent on presence of oxidized or reduced substrate | Pseudomonas fluorescens | |
| 1.6.1.2 | 2'-AMP | - |
Bos taurus | |
| 1.6.1.2 | 2'-AMP | - |
Mammalia | |
| 1.6.1.2 | 3'-5'-AMP | - |
Mammalia | |
| 1.6.1.2 | 3'-AMP | - |
Mammalia | |
| 1.6.1.2 | 5'-AMP | - |
Mammalia | |
| 1.6.1.2 | acetyl-CoA | - |
Mammalia | |
| 1.6.1.2 | acetyl-dephospho-CoA | competitive vs. NAD(H) | Mammalia | |
| 1.6.1.2 | adenosine | - |
Mammalia | |
| 1.6.1.2 | ADP | - |
Mammalia | |
| 1.6.1.2 | Ca2+ | - |
Bos taurus | |
| 1.6.1.2 | Ca2+ | - |
Mammalia | |
| 1.6.1.2 | CoA | - |
Bos taurus | |
| 1.6.1.2 | CoA | - |
Mammalia | |
| 1.6.1.2 | D2O | - |
Mammalia | |
| 1.6.1.2 | dephospho-CoA | competitive vs. NAD(H) | Mammalia | |
| 1.6.1.2 | Mg2+ | - |
Bos taurus | |
| 1.6.1.2 | Mg2+ | - |
Mammalia | |
| 1.6.1.2 | Mn2+ | - |
Bos taurus | |
| 1.6.1.2 | Mn2+ | - |
Mammalia | |
| 1.6.1.2 | palmitoyl-CoA | - |
Bos taurus | |
| 1.6.1.2 | palmitoyl-CoA | - |
Mammalia | |
| 1.6.1.2 | triiodothyronine | - |
Bos taurus | |
| 1.6.1.2 | triiodothyronine | - |
Mammalia | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.1.2 | 0.009 | - |
NADH | - |
Bos taurus | |
| 1.6.1.2 | 0.02 | - |
NADPH | - |
Bos taurus | |
| 1.6.1.2 | 0.028 | - |
NAD+ | - |
Bos taurus | |
| 1.6.1.2 | 0.04 | - |
NADP+ | - |
Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.1.2 | mitochondrial membrane | - |
Mammalia | 31966 | - |
| 1.6.1.2 | mitochondrial membrane | - |
Bos taurus | 31966 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | Ca2+ | - |
Pseudomonas fluorescens | |
| 1.6.1.1 | Ca2+ | - |
Pseudomonas aeruginosa | |
| 1.6.1.1 | Ca2+ | - |
Azotobacter vinelandii | |
| 1.6.1.1 | Ca2+ | - |
Azotobacter agilis | |
| 1.6.1.1 | Ca2+ | - |
Azotobacter chroococcum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.1.2 | NADH + NADP+ | Mammalia | - |
NADPH + NAD+ | - |
r | |
| 1.6.1.2 | NADH + NADP+ | Bos taurus | - |
NADPH + NAD+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.1.1 | Azotobacter agilis | - |
- |
- |
| 1.6.1.1 | Azotobacter chroococcum | - |
- |
- |
| 1.6.1.1 | Azotobacter vinelandii | - |
- |
- |
| 1.6.1.1 | Chromatium sp. | - |
- |
- |
| 1.6.1.1 | Pseudomonas aeruginosa | - |
- |
- |
| 1.6.1.1 | Pseudomonas fluorescens | - |
- |
- |
| 1.6.1.2 | Bos taurus | - |
- |
- |
| 1.6.1.2 | Mammalia | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.1.1 | overview | Pseudomonas fluorescens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.6.1.1 | NADPH + NAD+ = NADP+ + NADH | mechanism | Pseudomonas fluorescens | |
| 1.6.1.1 | NADPH + NAD+ = NADP+ + NADH | mechanism | Azotobacter agilis | |
| 1.6.1.1 | NADPH + NAD+ = NADP+ + NADH | mechanism | Azotobacter chroococcum | |
| 1.6.1.2 | NADPH + NAD+ = NADP+ + NADH | mechanism | Mammalia | |
| 1.6.1.2 | NADPH + NAD+ = NADP+ + NADH | mechanism | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.1.2 | artery | - |
Mammalia | - |
| 1.6.1.2 | artery | - |
Bos taurus | - |
| 1.6.1.2 | heart | - |
Mammalia | - |
| 1.6.1.2 | heart | - |
Bos taurus | - |
| 1.6.1.2 | kidney | - |
Bos taurus | - |
| 1.6.1.2 | liver | - |
Mammalia | - |
| 1.6.1.2 | liver | - |
Bos taurus | - |
| 1.6.1.2 | additional information | no or low activity in brain, prostate, seminal vesicle, spleen, testis | Mammalia | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 11.5 | - |
- |
Pseudomonas fluorescens |
| 1.6.1.1 | 24 | - |
- |
Chromatium sp. |
| 1.6.1.1 | 217 | - |
- |
Pseudomonas aeruginosa |
| 1.6.1.1 | 220 | 362 | - |
Azotobacter vinelandii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.1.1 | NADP+ + NADH | - |
Pseudomonas aeruginosa | NADPH + NAD+ | - |
r | |
| 1.6.1.1 | NADP+ + NADH | - |
Azotobacter vinelandii | NADPH + NAD+ | - |
r | |
| 1.6.1.1 | NADP+ + NADH | poorly reversible | Chromatium sp. | NADPH + NAD+ | - |
r | |
| 1.6.1.1 | NADP+ + NADH | degree of reversibility depends on source of enzyme | Pseudomonas fluorescens | NADPH + NAD+ | - |
r | |
| 1.6.1.1 | NADP+ + NADH | degree of reversibility depends on source of enzyme | Azotobacter agilis | NADPH + NAD+ | - |
r | |
| 1.6.1.1 | NADP+ + NADH | degree of reversibility depends on source of enzyme | Azotobacter chroococcum | NADPH + NAD+ | - |
r | |
| 1.6.1.1 | NADPH + 3-acetylpyridine-NAD+ | - |
Pseudomonas fluorescens | NADP+ + 3-acetylpyridine-NADH | - |
? | |
| 1.6.1.1 | NADPH + 3-acetylpyridine-NAD+ | - |
Pseudomonas aeruginosa | NADP+ + 3-acetylpyridine-NADH | - |
? | |
| 1.6.1.1 | NADPH + deamino-NAD+ | - |
Pseudomonas fluorescens | NADP+ + deamino-NADH | - |
? | |
| 1.6.1.1 | NADPH + deamino-NAD+ | - |
Pseudomonas aeruginosa | NADP+ + deamino-NADH | - |
? | |
| 1.6.1.1 | NADPH + deamino-NAD+ | - |
Azotobacter vinelandii | NADP+ + deamino-NADH | - |
? | |
| 1.6.1.1 | NADPH + pyridine aldehyde-NAD+ | - |
Pseudomonas fluorescens | NADP+ + pyridine aldehyde-NADH | - |
? | |
| 1.6.1.1 | NADPH + pyridine aldehyde-NAD+ | - |
Pseudomonas aeruginosa | NADP+ + pyridine aldehyde-NADH | - |
? | |
| 1.6.1.2 | NADH + NADP+ | specific for 4A site of NADH, i.e. pro-R hydrogen and 4B site of NADPH, i.e. pro-S hydrogen | Mammalia | NAD+ + NADPH | - |
? | |
| 1.6.1.2 | NADH + NADP+ | specific for 4A site of NADH, i.e. pro-R hydrogen and 4B site of NADPH, i.e. pro-S hydrogen | Bos taurus | NAD+ + NADPH | - |
? | |
| 1.6.1.2 | NADH + NADP+ | inactive against 3'-analogs of NADP+ | Mammalia | NAD+ + NADPH | - |
? | |
| 1.6.1.2 | NADH + NADP+ | - |
Mammalia | NADPH + NAD+ | - |
r | |
| 1.6.1.2 | NADH + NADP+ | - |
Bos taurus | NADPH + NAD+ | - |
r | |
| 1.6.1.2 | NADH + thio-NADP+ | - |
Mammalia | NAD+ + thio-NADPH | - |
r | |
| 1.6.1.2 | NADH + thio-NADP+ | - |
Bos taurus | NAD+ + thio-NADPH | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 7 | 8 | - |
Pseudomonas fluorescens |
| 1.6.1.1 | 7 | 8 | - |
Azotobacter vinelandii |
| 1.6.1.1 | 7 | 8 | - |
Azotobacter agilis |
| 1.6.1.1 | 7 | 8 | - |
Azotobacter chroococcum |
| 1.6.1.2 | 5.5 | - |
reduction of NADP+ | Mammalia |
| 1.6.1.2 | 7 | - |
- |
Bos taurus |
| 1.6.1.2 | 7 | - |
reduction of NAD+ | Mammalia |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | FAD | inactivation by heat treatment can be reversed by addition of FAD | Pseudomonas fluorescens | |
| 1.6.1.1 | FAD | inactivation by heat treatment can be reversed by addition of FAD | Pseudomonas aeruginosa | |
| 1.6.1.1 | FAD | inactivation by heat treatment can be reversed by addition of FAD | Azotobacter vinelandii | |
| 1.6.1.1 | FAD | inactivation by heat treatment can be reversed by addition of FAD | Azotobacter agilis | |
| 1.6.1.1 | FAD | inactivation by heat treatment can be reversed by addition of FAD | Azotobacter chroococcum | |
| 1.6.1.2 | NAD+ | - |
Mammalia | |
| 1.6.1.2 | NAD+ | - |
Bos taurus | |
| 1.6.1.2 | NADH | - |
Mammalia | |
| 1.6.1.2 | NADH | - |
Bos taurus | |
| 1.6.1.2 | NADP+ | - |
Mammalia | |
| 1.6.1.2 | NADP+ | - |
Bos taurus | |
| 1.6.1.2 | NADPH | - |
Mammalia | |
| 1.6.1.2 | NADPH | - |
Bos taurus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.1.2 | 0.00015 | - |
palmitoyl-CoA | - |
Mammalia | |
| 1.6.1.2 | 0.009 | - |
dephospho-CoA | - |
Mammalia | |
| 1.6.1.2 | 0.011 | - |
acetyl-dephospho-CoA | - |
Mammalia | |
| 1.6.1.2 | 0.2 | - |
CoA | - |
Mammalia | |
| 1.6.1.2 | 0.2 | - |
CoA | - |
Bos taurus | |
| 1.6.1.2 | 0.2 | - |
acetyl-CoA | - |
Mammalia | |
| 1.6.1.2 | 0.3 | - |
ADP | - |
Mammalia | |
| 1.6.1.2 | 0.3 | - |
5'-AMP | - |
Mammalia | |
| 1.6.1.2 | 0.4 | - |
3'-5'-AMP | - |
Mammalia | |
| 1.6.1.2 | 0.5 | - |
adenosine | - |
Mammalia | |
| 1.6.1.2 | 0.7 | - |
3'-AMP | - |
Mammalia | |
| 1.6.1.2 | 0.7 | - |
2'-AMP | - |
Mammalia | |
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