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Literature summary extracted from

  • Voordouw, G.; van der Vies, S.; Scholten, J.W.; Veeger, C.
    Pyridine nucleotide transhydrogenase from Azotobacter vinelandii. Differences in properties between the purified and the cell-free extract enzyme (1980), Eur. J. Biochem., 107, 337-344.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.1.1 p-Aminophenylarsenoxide 0.1 mM, 40-60% inhibition in the absence of either phosphate or magnesium ions, reduction of NAD+ by NADPH in cell-free extracts is rapidly and completely inhibited in the presence of 20 mM phosphate Azotobacter vinelandii
1.6.1.1 p-chloromercuribenzoate 0.044 mM, 40-50% inhibition after 30 min, activity can be restored by adding 2-mercaptoethanol Azotobacter vinelandii
1.6.1.1 p-hydroxymercuribenzoate
-
 Azotobacter vinelandii
1.6.1.1 phosphate 10-25 mM, 60-70% inhibition of purified enzyme, complete inhibition of enzyme in cell-free extracts by 5-10 mM phosphate, NADP+ reduction by NADH is inhibited, reduction of NAD+ by NADPH is hardly affected Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.1.1 Ca2+ 10-20fold activation of NAD+ reduction by NADPH at alkaline pH in cell-free extracts Azotobacter vinelandii
1.6.1.1 Mg2+ 10-20fold activation of NAD+ reduction by NADPH at alkaline pH in cell-free extracts Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.1.1 421000
-
 purified enzyme: exceptional stability of polymers at neutral pH Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.1 Azotobacter vinelandii
-
-
-

Subunits

EC Number Subunits Comment Organism
1.6.1.1 octamer octameric in cell-free extract, polymeric in purified form Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.1.1 7 8
-
 Azotobacter vinelandii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.1.1 8.4 8.7 half maximal activity in this pH range in the absence of Ca2+ or Mg2+, no activity above Azotobacter vinelandii
1.6.1.1 9.1 9.3 half maximal activity in this pH range in the presence of 5 mM Ca2+ or Mg2+, no activity above Azotobacter vinelandii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.6.1.1 7
-
 polymeric enzyme stable Azotobacter vinelandii