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Literature summary extracted from

  • Wittwer, A.J.; Wagner, C.
    Identification of folate binding protein of mitochondria as dimethylglycine dehydrogenase (1980), Proc. Natl. Acad. Sci. USA, 77, 4484-4488.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.8.4 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.8.4 90000
-
 x * 90000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.4 N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor Rattus norvegicus during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
 ?
1.5.8.4 N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor Rattus norvegicus reaction of the choline degradation pathway sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.4 using gel filtration chromatography on Sephadex G-150, DEAE-cellulose chromatography, and affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.8.4 82.3
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.4 N,N-dimethylglycine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Rattus norvegicus sarcosine + formaldehyde + reduced acceptor
-
 ?
1.5.8.4 N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate Rattus norvegicus sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
 ?
1.5.8.4 N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor reaction of the choline degradation pathway Rattus norvegicus sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.8.4 ? x * 90000, SDS-PAGE Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.4 FAD covalently bound Rattus norvegicus
1.5.8.4 Tetrahydropteroylpentaglutamate
-
 Rattus norvegicus