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Literature summary extracted from

  • Kinzel, J.J.; Bhattacharjee, J.K.
    Lysine biosynthesis in Rhodotorula glutinis: properties of pipecolic acid oxidase (1982), J. Bacteriol., 151, 1073-1077.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.7 HgCl2 100% inhibition at 1 mM Rhodotorula glutinis
1.5.3.7 hydroxylamine 15% inhibition at 1 mM Rhodotorula glutinis
1.5.3.7 KCN 20% inhibition at 1 mM Rhodotorula glutinis
1.5.3.7 L-proline competitive inhibitor Rhodotorula glutinis
1.5.3.7 p-chloromercuribenzoate 100% inhibition at 1 mM Rhodotorula glutinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.7 1.7
-
 L-pipecolic acid
-
 Rhodotorula glutinis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.7 43000
-
 1 * 43000, SDS-PAGE Rhodotorula glutinis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.7 L-pipecolate + O2 Rhodotorula glutinis
-
 2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2 the product reacts with water to form 2-aminoadipate 6-semialdehyde ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.7 Rhodotorula glutinis
-
 strains wild-type, MU-1 and lys5
-

Storage Stability

EC Number Storage Stability Organism
1.5.3.7 -70°C, Tris-HCl buffer pH 8.7, 1 mM 2-mercaptoethanol, several weeks, 100% activity Rhodotorula glutinis
1.5.3.7 4°C, Tris-HCl buffer pH 8.7, 1 mM 2-mercaptoethanol, several days, 100% activity Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.7 L-pipecolate + O2
-
 Rhodotorula glutinis 2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
 ?
1.5.3.7 L-pipecolate + O2
-
 Rhodotorula glutinis 2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2 the product reacts with water to form 2-aminoadipate 6-semialdehyde ?

Subunits

EC Number Subunits Comment Organism
1.5.3.7 monomer 1 * 43000, SDS-PAGE Rhodotorula glutinis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.7 45
-
 10 min 20% activity, bovine serum albumin at 1 mg per ml protects from thermal inactivation Rhodotorula glutinis
1.5.3.7 53
-
 complete inactivation Rhodotorula glutinis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.7 8.5
-
-
 Rhodotorula glutinis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.3.7 11
-
 L-proline
-
 Rhodotorula glutinis