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Literature summary extracted from

  • Ogushi, S.; Nagao, K.; Emi, S.; Ando, M.; Tsuru, D.
    Sarcosine oxidase from Arthrobacter urefaciens: Purification and some properties (1988), Chem. Pharm. Bull., 36, 1445-1450.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.1 2-mercaptoethanol slight Paenarthrobacter ureafaciens
1.5.3.1 Cd2+
-
 Paenarthrobacter ureafaciens
1.5.3.1 dithiothreitol
-
 Paenarthrobacter ureafaciens
1.5.3.1 EDTA slight Paenarthrobacter ureafaciens
1.5.3.1 iodoacetamide
-
 Paenarthrobacter ureafaciens
1.5.3.1 iodoacetate
-
 Paenarthrobacter ureafaciens
1.5.3.1 N-ethylmaleimide
-
 Paenarthrobacter ureafaciens
1.5.3.1 o-phenanthroline
-
 Paenarthrobacter ureafaciens
1.5.3.1 p-chloromercuribenzoate
-
 Paenarthrobacter ureafaciens
1.5.3.1 Zn2+
-
 Paenarthrobacter ureafaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.1 6.4
-
 sarcosine
-
 Paenarthrobacter ureafaciens
1.5.3.1 16.5
-
 N-Methyl-DL-alanine
-
 Paenarthrobacter ureafaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.1 14000
-
 1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens
1.5.3.1 23000
-
 1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens
1.5.3.1 45000
-
 1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens
1.5.3.1 96000
-
 1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens
1.5.3.1 185000
-
 gel filtration Paenarthrobacter ureafaciens

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Paenarthrobacter ureafaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.1
-
 Paenarthrobacter ureafaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.1 7.3
-
-
 Paenarthrobacter ureafaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 N-methyl-DL-alanine + O2 + H2O
-
 Paenarthrobacter ureafaciens formaldehyde + DL-alanine + H2O2
-
 ?
1.5.3.1 sarcosine + O2 + H2O
-
 Paenarthrobacter ureafaciens glycine + formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.1 tetramer 1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.1 20
-
 pH 6.5-9.5, 20 h Paenarthrobacter ureafaciens
1.5.3.1 40
-
 pH 8.0, 24 h, 50% loss of activity Paenarthrobacter ureafaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.1 4.1
-
 N-Methyl-DL-alanine
-
 Paenarthrobacter ureafaciens
1.5.3.1 5.8
-
 sarcosine
-
 Paenarthrobacter ureafaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.1 8.3
-
-
 Paenarthrobacter ureafaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.1 6 10 pH 6: about 35% of maximum activity Paenarthrobacter ureafaciens
1.5.3.1 6 10 pH 10: about 55% of maximum activity Paenarthrobacter ureafaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.3.1 6.5 9.5 20°C, stable for 20 h Paenarthrobacter ureafaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.1 flavin associated with 45000 Da subunit Paenarthrobacter ureafaciens