Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Krupenko, S.A.; Wagner, C.; Cook, R.J.
    Cysteine 707 is involved in the dehydrogenase active site of rat 10-formyltetrahydrofolate dehydrogenase (1995), J. Biol. Chem., 270, 519-522.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.6 expression of FDH cDNA, subcloned in the pVL 1393 baculovirus vector, in Sf9 insect cells, using the MaxBac expression system Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.6 C707A mutant with a complete loss of dehydrogenase activity of 10-formyltetrahydrofolate and propanal, but hydrolase activity and binding of NADP+ is unchanged Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.6 99000
-
 4 * 99000, wild-type FDH and C707A mutant Rattus norvegicus
1.5.1.6 393200
-
 C707A mutant FDH, molecular size detector employing laser-light scattering methodology Rattus norvegicus
1.5.1.6 398800
-
 wild-type FDH, molecular size detector employing laser-light scattering methodology Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6 purification of a C707A mutant of FDH Rattus norvegicus
1.5.1.6 purification of recombinant liver FDH Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.6 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyl-5,8-dideazafolate + NADP+ + H2O
-
 Rattus norvegicus 5,8-dideazafolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O Cys-707 is a key residue of the dehydrogenase active site and acts as nucleophile in the formation of an enzyme-linked thiohemiacetal intermediate Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 additional information enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate Rattus norvegicus ?
-
 ?
1.5.1.6 additional information 10-formyltetrahydrofolate dehydrogenase/hydrolase activities occur at the same time and are associated with separate active sites Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.6 homotetramer 4 * 99000, wild-type FDH and C707A mutant Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
1.5.1.6 NADP+ NADP+-dependent Rattus norvegicus
1.5.1.6 tetrahydrofolate
-
 Rattus norvegicus