Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, S.; Park, G.H.; Joo, W.A.; Paik, W.K.; Cook, R.J.; Williams, K.R.
    Identification of protein-arginine N-methyltransferase as 10-formyltetrahydrofolate dehydrogenase (1998), J. Biol. Chem., 273, 27374-27382.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.6 2-mercaptoethanol optimal dehydrogenase activity requires 15 mM or higher concentrations Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.6 expression of FDH in insect cells using the pVL 1393 baculovirus expression vector Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.6 additional information recombinant FDH is not inhibited by sodium azide Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.6 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.6 106000
-
 4 * 106000, SDS-PAGE Rattus norvegicus
1.5.1.6 450000
-
 nondenaturing PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O Rattus norvegicus regulatory mechanism to control the in vivo folate pool size tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6 purification of recombinant liver FDH Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.6 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.6 0.094
-
 recombinant FDH, 10-formyltetrahydrofolate as substrate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyl-5,8-dideazafolate + NADP+ + H2O
-
 Rattus norvegicus 5,8-dideazafolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O
-
 Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O regulatory mechanism to control the in vivo folate pool size Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 additional information enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate Rattus norvegicus ?
-
 ?
1.5.1.6 additional information FDH has also methyltransferase activity Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.6 homotetramer 4 * 106000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.5.1.6 More identical with protein-arginine methyltransferase from liver cytosol, EC 2.1.1.23 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
1.5.1.6 folate 10-formyl-5,8-dideazafolate and 5,8-dideazafolate Rattus norvegicus
1.5.1.6 NADP+ NADP+-dependent Rattus norvegicus
1.5.1.6 tetrahydrofolate
-
 Rattus norvegicus