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Literature summary extracted from

  • Krupenko, S.A.; Vlasov, A.P.; Wagner, C.
    On the role of conserved histidine 106 in 10-formyltetrahydrofolate dehydrogenase catalysis (2001), J. Biol. Chem., 276, 24030-24037.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.6 expression of H106K mutant of FDH in insect cells SF9, using a baculovirus expression system, and of N-terminal domain mutants in Escherichia coli BL 21 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.6 H106A insoluble recombinant protein Rattus norvegicus
1.5.1.6 H106K mutant with a complete loss of 10-formyltetrahydrofolate dehydrogenase and hydrolase activity, aldehyde dehydrogenase activity is similar to wild-type FDH Rattus norvegicus
3.5.1.10 H100A soluble, shows enzymic activity Rattus norvegicus
3.5.1.10 H106A insoluble, no enzymatic activity Rattus norvegicus
3.5.1.10 H106E insoluble, no enzymatic activity Rattus norvegicus
3.5.1.10 H106K insoluble, no enzymatic activity Rattus norvegicus
3.5.1.10 H106N insoluble, no enzymatic activity Rattus norvegicus
3.5.1.10 H106Q insoluble, no enzymatic activity Rattus norvegicus
3.5.1.10 H106R insoluble, no enzymatic activity Rattus norvegicus
3.5.1.10 H113A insoluble, shows enzymic activity Rattus norvegicus
3.5.1.10 H125A soluble, shows enzymic activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.10 0.007
-
10-formyltetrahydrofolate 30°C, pH 7.8, wild type Rattus norvegicus
3.5.1.10 0.0083
-
10-formyltetrahydrofolate 30°C, pH 7.8, H125A mutant Rattus norvegicus
3.5.1.10 0.009
-
10-formyltetrahydrofolate 30°C, pH 7.8, H113A mutant Rattus norvegicus
3.5.1.10 0.0095
-
10-formyltetrahydrofolate 30°C, pH 7.8, H100A mutant Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.6 cytosol
-
Rattus norvegicus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Rattus norvegicus
-
-
-
3.5.1.10 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6 purification of N-terminal domain mutants and of a H106K mutant of FDH Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ reaction mechanism Rattus norvegicus
3.5.1.10 10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate dehydrogenase reaction requires full length enzyme, hydrolase reaction occurs in amino-terminal domain of enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.6 liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O His-106 is involved in enzyme catalysis and in folding of the N-terminal domain, enzyme requires Cys-707 for catalysis, which is located inside the C-terminal domain, mechanism includes hydrolase reaction as essential part Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?
1.5.1.6 additional information 310 amino acid residue N-terminal domain has 10-formyltetrahydrofolate hydrolase activity and substrate binding site, C-terminal domain has aldehyde dehydrogenase activity and is used as catalytic center in dehydrogenase reaction, full-length enzyme is required for 10-formyltetrahydrofolate dehydrogenase activity, the two domains work in concert Rattus norvegicus ?
-
?
3.5.1.10 10-formyltetrahydrofolate + H2O
-
Rattus norvegicus formate + tetrahydrofolate
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.6 homotetramer monomer with 902 amino acid residues Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.6 30
-
assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
1.5.1.6 NADP+ NADP+-dependent Rattus norvegicus
1.5.1.6 tetrahydrofolate
-
Rattus norvegicus
3.5.1.10 NADP+ required for dehydrogenase reaction Rattus norvegicus