EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.6 | expression of H106K mutant of FDH in insect cells SF9, using a baculovirus expression system, and of N-terminal domain mutants in Escherichia coli BL 21 | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.6 | H106A | insoluble recombinant protein | Rattus norvegicus |
1.5.1.6 | H106K | mutant with a complete loss of 10-formyltetrahydrofolate dehydrogenase and hydrolase activity, aldehyde dehydrogenase activity is similar to wild-type FDH | Rattus norvegicus |
3.5.1.10 | H100A | soluble, shows enzymic activity | Rattus norvegicus |
3.5.1.10 | H106A | insoluble, no enzymatic activity | Rattus norvegicus |
3.5.1.10 | H106E | insoluble, no enzymatic activity | Rattus norvegicus |
3.5.1.10 | H106K | insoluble, no enzymatic activity | Rattus norvegicus |
3.5.1.10 | H106N | insoluble, no enzymatic activity | Rattus norvegicus |
3.5.1.10 | H106Q | insoluble, no enzymatic activity | Rattus norvegicus |
3.5.1.10 | H106R | insoluble, no enzymatic activity | Rattus norvegicus |
3.5.1.10 | H113A | insoluble, shows enzymic activity | Rattus norvegicus |
3.5.1.10 | H125A | soluble, shows enzymic activity | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.10 | 0.007 | - |
10-formyltetrahydrofolate | 30°C, pH 7.8, wild type | Rattus norvegicus | |
3.5.1.10 | 0.0083 | - |
10-formyltetrahydrofolate | 30°C, pH 7.8, H125A mutant | Rattus norvegicus | |
3.5.1.10 | 0.009 | - |
10-formyltetrahydrofolate | 30°C, pH 7.8, H113A mutant | Rattus norvegicus | |
3.5.1.10 | 0.0095 | - |
10-formyltetrahydrofolate | 30°C, pH 7.8, H100A mutant | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.1.6 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.6 | Rattus norvegicus | - |
- |
- |
3.5.1.10 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.6 | purification of N-terminal domain mutants and of a H106K mutant of FDH | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ | reaction mechanism | Rattus norvegicus | |
3.5.1.10 | 10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate | dehydrogenase reaction requires full length enzyme, hydrolase reaction occurs in amino-terminal domain of enzyme | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.6 | liver | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | His-106 is involved in enzyme catalysis and in folding of the N-terminal domain, enzyme requires Cys-707 for catalysis, which is located inside the C-terminal domain, mechanism includes hydrolase reaction as essential part | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
1.5.1.6 | additional information | 310 amino acid residue N-terminal domain has 10-formyltetrahydrofolate hydrolase activity and substrate binding site, C-terminal domain has aldehyde dehydrogenase activity and is used as catalytic center in dehydrogenase reaction, full-length enzyme is required for 10-formyltetrahydrofolate dehydrogenase activity, the two domains work in concert | Rattus norvegicus | ? | - |
? | |
3.5.1.10 | 10-formyltetrahydrofolate + H2O | - |
Rattus norvegicus | formate + tetrahydrofolate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.6 | homotetramer | monomer with 902 amino acid residues | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.6 | 30 | - |
assay at | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.6 | 10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
1.5.1.6 | NADP+ | NADP+-dependent | Rattus norvegicus | |
1.5.1.6 | tetrahydrofolate | - |
Rattus norvegicus | |
3.5.1.10 | NADP+ | required for dehydrogenase reaction | Rattus norvegicus |