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Literature summary extracted from
- Enzymatic activities of rat liver cytosol 10-formyltetrahydrofolate dehydrogenase (1995), Arch. Biochem. Biophys., 321, 336-344.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.6 | 2-mercaptoethanol | 5 to 15 mM: 1.7fold increase in activity, higher concentrations have no further effect, it may reduce oxidized Cys-707 or another important sulfhydryl group | Rattus norvegicus |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.1.6 | clone expressed in insect cells has both dehydrogenase and hydrolase activity and exhibits almost identical kinetic parameters to the native rat liver enzyme | Rattus norvegicus |
| 1.5.1.6 | FDH is cloned | Rattus norvegicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.5.1.6 | C707A | mutant with a complete loss of NADP+-dependent 10-formyltetrahydrofolate dehydrogenase activity, but no influence on hydrolase activity | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.6 | 5,10-dideazatetrahydrofolate | 0.4 mM, 98% inhibition, folate analog | Rattus norvegicus | |
| 1.5.1.6 | additional information | not inhibited by the folate analog N-[4-[[3-(2,4-diamino-1,6-dihydro-6-oxo-5-pyrimidinyl)propyl]amino]-benzoyl]-L-glutamate | Rattus norvegicus | |
| 1.5.1.6 | N-ethylmaleimide | 1 mM or 10 mM, complete inhibition | Rattus norvegicus | |
| 1.5.1.6 | propanal | FDH activity decreases with increasing concentrations, competes with 10-formyltetrahydrofolate for NADP+-dependent oxidation | Rattus norvegicus | |
| 1.5.1.6 | tetrahydrofolate | - |
Oryctolagus cuniculus | |
| 1.5.1.6 | tetrahydrofolate | - |
Rattus norvegicus | |
| 1.5.1.6 | Trypsin | - |
Oryctolagus cuniculus | |
| 1.5.1.6 | Trypsin | tryptic digestion inactivates 10-formyltetrahydrofolate dehydrogenase but not hydrolase activity | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.6 | 0.001 | - |
NADP+ | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.5.1.6 | cytosol | - |
Rattus norvegicus | 5829 | - |
| 1.5.1.6 | cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.6 | 99015 | - |
x * 99015, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.6 | additional information | Rattus norvegicus | enzyme functions not as an aldehyde dehydrogenase in vivo, 10-formyltetrahydrofolate hydrolase activity is of no physiological significance, disulfiram may inhibit enzyme activity and probably perturb hepatic folate metabolism in vivo | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.6 | Oryctolagus cuniculus | - |
- |
- |
| 1.5.1.6 | Rattus norvegicus | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.5.1.6 | prone to oxidative inactivation, but may be partially reactivated by incubation with 100 mM 2-mercaptoethanol or 100 mM dithiothreitol | Rattus norvegicus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.6 | - |
Rattus norvegicus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ | reaction mechanism | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.1.6 | liver | - |
Rattus norvegicus | - |
| 1.5.1.6 | liver | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.6 | 10-formyl-5,8-dideazafolate + NADP+ + H2O | - |
Rattus norvegicus | 5,8-dideazafolate + CO2 + NADPH + H+ | - |
? | |
| 1.5.1.6 | 10-formyl-5,8-dideazafolate + NADP+ + H2O | - |
Oryctolagus cuniculus | 5,8-dideazafolate + CO2 + NADPH + H+ | - |
? | |
| 1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | - |
Oryctolagus cuniculus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
| 1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
| 1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | mechanism which proceeds through thiohemiacetal and thioester intermediates, nucleophilic attack by the essential active site Cys-707 on the carbonyl group of 10-formyltetrahydrofolate, folate binding domain requires presence of an intact ring system | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
| 1.5.1.6 | additional information | enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate | Rattus norvegicus | ? | - |
? | |
| 1.5.1.6 | additional information | enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate | Oryctolagus cuniculus | ? | - |
? | |
| 1.5.1.6 | additional information | enzyme catalyzes also NADP+-independent hydrolytic cleavage of 10-formyltetrahydrofolate to tetrahydrofolate and formate, hydrolysis is very inefficient compared to dehydrogenase reaction and may be an artifact of assay system | Rattus norvegicus | ? | - |
? | |
| 1.5.1.6 | additional information | enzyme functions not as an aldehyde dehydrogenase in vivo, 10-formyltetrahydrofolate hydrolase activity is of no physiological significance, disulfiram may inhibit enzyme activity and probably perturb hepatic folate metabolism in vivo | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.1.6 | ? | x * 99015, SDS-PAGE | Rattus norvegicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.1.6 | More | identical with folate binding protein cytosol-I, FBP-CI | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.6 | 30 | - |
assay at | Rattus norvegicus |
| 1.5.1.6 | 30 | - |
assay at | Oryctolagus cuniculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.6 | 10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
| 1.5.1.6 | 10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Oryctolagus cuniculus | |
| 1.5.1.6 | folate | 10-formyl-5,8-dideazafolate and 5,8-dideazafolate | Rattus norvegicus | |
| 1.5.1.6 | folate | 10-formyl-5,8-dideazafolate and 5,8-dideazafolate | Oryctolagus cuniculus | |
| 1.5.1.6 | NADP+ | NADP+-dependent | Rattus norvegicus | |
| 1.5.1.6 | NADP+ | NADP+-dependent | Oryctolagus cuniculus | |
| 1.5.1.6 | NADP+ | binding of substrate, 10-formyltetrahydrofolate or propanal, influences enzyme affinity for NADP+ | Rattus norvegicus | |
| 1.5.1.6 | tetrahydrofolate | - |
Rattus norvegicus | |
| 1.5.1.6 | tetrahydrofolate | - |
Oryctolagus cuniculus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.6 | 0.048 | - |
5,10-dideazatetrahydrofolate | - |
Rattus norvegicus | |
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