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Literature summary extracted from

  • Albrecht, A.M.; Pearce, F.K.; Hutchinson, D.J.
    Methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A and its repressibility by serine (1968), J. Bacteriol., 95, 1779-1789.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.5.1.5 extremely labile, during purification protection by NADP+ Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.5 guanine 48% inhibition Enterococcus faecium
1.5.1.5 Iodosobenzoate
-
 Enterococcus faecium
1.5.1.5 Mg2+ inhibition of 5% Enterococcus faecium
1.5.1.5 N-ethylmaleimide
-
 Enterococcus faecium
1.5.1.5 p-chloromercuribenzoate
-
 Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.021
-
 NADP+
-
 Enterococcus faecium
1.5.1.5 0.026
-
 5,10-methylenetetrahydrofolate
-
 Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Enterococcus faecium
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Enterococcus faecium
-
 var.durans Ak, an amethopterin-resistent mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.5 partial Enterococcus faecium

Storage Stability

EC Number Storage Stability Organism
1.5.1.5 -20°C, 0.1% bovine plasma albumin Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Enterococcus faecium 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.5 7.2
-
-
 Enterococcus faecium

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NADP+
-
 Enterococcus faecium