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Literature summary extracted from

  • Wang, H.; Lei, B.; Tu, S.C.
    Vibrio harveyi NADPH-FMN oxidoreductase Arg203 as a critical residue for NADPH recognition and binding (2000), Biochemistry, 39, 7813-7819.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.30 expressed in Escherichia coli Vibrio harveyi

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.30 R203A decrease of NADPH binding potential, critical role of Arg203 in the specific recognition and binding of NADPH Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.30 0.0058
-
 FMN flavin reductase P R203A Vibrio harveyi
1.5.1.30 0.0069
-
 FMN native flavin reductase P Vibrio harveyi
1.5.1.30 0.021
-
 NADPH native flavin reductase P Vibrio harveyi
1.5.1.30 0.71
-
 NADPH flavin reductase P R203A Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Vibrio harveyi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FMN + NADPH
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 NADPH:FMN oxidoreductase
-
 Vibrio harveyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.30 53.8
-
 NADPH flavin reductase P R203A Vibrio harveyi
1.5.1.30 69.5
-
 NADPH native flavin reductase P Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 FMN
-
 Vibrio harveyi
1.5.1.30 NADPH
-
 Vibrio harveyi