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Literature summary extracted from

  • Lei, B.; Liu, M.; Huang, S.; Tu, S.C.
    Vibrio harveyi NADPH-flavin oxidoreductase: cloning, sequencing and overexpression of the gene and purification and characterization of the cloned enzyme (1994), J. Bacteriol., 176, 3552-3558.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.30 expressed in Escherichia coli Vibrio harveyi
1.5.1.38 overexpression in Escherichia coli Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.30 0.007
-
 FMN
-
 Vibrio harveyi
1.5.1.30 0.02
-
 NADPH
-
 Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.30 26320
-
 calculated from sequence of cloned DNA Vibrio harveyi
1.5.1.30 28000
-
 1 * 28000, wild type and cloned, SDS-PAGE Vibrio harveyi
1.5.1.30 33000
-
 gel filtration Vibrio harveyi
1.5.1.38 26312
-
 1 * 26312, calculated from sequence Vibrio harveyi
1.5.1.38 28000
-
 1 * 28000, SDS-PAGE Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.38 FMN + NADPH + H+ Vibrio harveyi
-
 FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+ Vibrio harveyi MAV / ATCC 33843
-
 FMNH2 + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Vibrio harveyi
-
-
-
1.5.1.30 Vibrio harveyi MAV / ATCC 33843
-
-
-
1.5.1.38 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.30 FMN-agarose chromatography as a key step Vibrio harveyi
1.5.1.38
-
 Vibrio harveyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.30 88
-
-
 Vibrio harveyi
1.5.1.38 88.4
-
 23°C, pH not specified in the publication Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FMN + NADPH
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.30 FMN + NADPH
-
 Vibrio harveyi MAV / ATCC 33843 FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+
-
 Vibrio harveyi MAV / ATCC 33843 FMNH2 + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.30 monomer 1 * 28000, wild type and cloned, SDS-PAGE Vibrio harveyi
1.5.1.38 monomer 1 * 28000, SDS-PAGE Vibrio harveyi
1.5.1.38 monomer 1 * 26312, calculated from sequence Vibrio harveyi

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 NADPH:flavin oxidoreductase
-
 Vibrio harveyi
1.5.1.38 flavin reductase P
-
 Vibrio harveyi
1.5.1.38 FRP
-
 Vibrio harveyi
1.5.1.38 NADPH-flavin oxidoreductase
-
 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.30 23
-
 enzyme assay Vibrio harveyi
1.5.1.38 23
-
 assay at Vibrio harveyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.30 36
-
 FMN
-
 Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 FMN tightly bound Vibrio harveyi
1.5.1.38 FMN contains a tightly bound FMN cofactor Vibrio harveyi