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Literature summary extracted from

  • Wilquet, V.; Gaspar, J.A.; van de Lande, M.; van de Casteele, M.; Legrain, C.; Meiering, E.M.; Glansdorff, N.
    Purification and characterization of recombinant Thermotoga maritima dihydrofolate reductase (1998), Eur. J. Biochem., 255, 628-637.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.3 KCl recombinant enzyme, 1.4fold activation at 40-200 mM Thermotoga maritima
1.5.1.3 NaCl recombinant enzyme, 1.4fold activation at 40-200 mM Thermotoga maritima
1.5.1.3 Urea recombinant enzyme, 7 M Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expression in Escherichia coli Thermotoga maritima

General Stability

EC Number General Stability Organism
1.5.1.3 substrates 7,8-dihydrofolate and NADPH stabilize against thermal inactivation Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 Ca2+ recombinant enzyme, above 20 mM Thermotoga maritima
1.5.1.3 Guanidine-HCl recombinant enzyme, 0.5 M Thermotoga maritima
1.5.1.3 methotrexate recombinant enzyme Thermotoga maritima
1.5.1.3 Sodium dodecyl sulfate recombinant enzyme, 0.1 M Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0003
-
 7,8-dihydrofolate recombinant enzyme Thermotoga maritima
1.5.1.3 0.004
-
 NADPH recombinant enzyme Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 19000
-
 2 * 19000, recombinant enzyme, SDS-PAGE, mass spectroscopy Thermotoga maritima
1.5.1.3 36000
-
 about, recombinant enzyme, gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Thermotoga maritima
-
 thermophilic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 recombinant enzyme from Escherichia coli Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 173
-
 recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Thermotoga maritima 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 dimer 2 * 19000, recombinant enzyme, SDS-PAGE, mass spectroscopy Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 37
-
 assay at Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 80
-
 half-life: about 60 min, half-life with NADPH: more than 360 min, half-life with 7,8-dihydrofolate: more than 240 min, half-life with 1M urea: 26-28 min Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 additional information
-
 enzyme-dihydrofolate complex is soluble at basic pH and precipitates reversibly at acidic pH Thermotoga maritima
1.5.1.3 6.3
-
 recombinant enzyme, 60°C Thermotoga maritima
1.5.1.3 6.5
-
 recombinant enzyme, 37°C Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Thermotoga maritima