Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Walker, V.K.; Tyshenko, M.G.; Kuiper, M.J.; Dargar, R.V.; Yuhas, D.A.; Cruickshank, P.A.; Chaguturu, R.
    Tobacco budworm dihydrofolate reductase is a promising target for insecticide discovery (2000), Eur. J. Biochem., 267, 394-402.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.3 dithiothreitol
-
 Heliothis virescens
1.5.1.3 p-chloromercuribenzoate
-
 Heliothis virescens

Application

EC Number Application Comment Organism
1.5.1.3 medicine potentially target for the discovery of novel insecticides and anthelminthics Heliothis virescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 amplification and DNA-sequencing of full length gene from DNA-library Heliothis virescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate complete inhibition at 0.0001 mM, slow tight binding inhibitor Heliothis virescens
1.5.1.3 p-hydroxymercuribenzoate
-
 Heliothis virescens
1.5.1.3 pyrimethamine anti-malarial DHFR inhibitor Heliothis virescens
1.5.1.3 Sodium tetrathionate thiol-blocking reagent Heliothis virescens
1.5.1.3 trimethoprim
-
 Heliothis virescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.3 soluble
-
 Drosophila melanogaster
-
-
1.5.1.3 soluble at low levels in the cytosol Heliothis virescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 19000 21000 DNA-sequence dtermination and SDS-PAGE Heliothis virescens

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Drosophila melanogaster
-
-
-
1.5.1.3 Heliothis virescens
-
 i.e. Helicoverpa virescens, tobacco budworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Drosophila melanogaster
1.5.1.3
-
 Heliothis virescens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ substrate and antifolate inhibitor methotrexate binding, modeling of three-dimensional structure Heliothis virescens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 larva
-
 Heliothis virescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 20.8
-
 purified enzyme Heliothis virescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH strongly specific for Drosophila melanogaster 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH strongly specific for Heliothis virescens 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH no activity Drosophila melanogaster 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH no activity Heliothis virescens 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH specific Drosophila melanogaster
1.5.1.3 NADPH specific Heliothis virescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.0000003
-
 methotrexate
-
 Heliothis virescens
1.5.1.3 0.000021
-
 pyrimethamine
-
 Heliothis virescens
1.5.1.3 0.00024
-
 trimethoprim
-
 Heliothis virescens