Literature summary extracted from

Cinquina, C.C.; Grogan, E.; Sun, R.; Lin, S.F.; Beardsley, G.P.; Miller, G.
Dihydrofolate reductase from Kaposi's sarcoma-associated herpesvirus (2000), Virology, 268, 201-217.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.5.1.3	overexpression in Escherichia coli	Human gammaherpesvirus 8

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.5.1.3	additional information	C-terminal-truncated mutant with lower activity	Human gammaherpesvirus 8

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.5.1.3	7,8-dihydrofolate	substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme	Human gammaherpesvirus 8
1.5.1.3	aminopterin	-	Homo sapiens
1.5.1.3	aminopterin	-	Human gammaherpesvirus 8
1.5.1.3	methotrexate	recombinant enzyme	Homo sapiens
1.5.1.3	methotrexate	-	Human gammaherpesvirus 8
1.5.1.3	piritrexim	recombinant enzyme	Homo sapiens
1.5.1.3	piritrexim	-	Human gammaherpesvirus 8
1.5.1.3	pyrimethamine	recombinant enzyme	Homo sapiens
1.5.1.3	pyrimethamine	-	Human gammaherpesvirus 8
1.5.1.3	trimethoprim	recombinant enzyme	Homo sapiens
1.5.1.3	trimethoprim	-	Human gammaherpesvirus 8

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5.1.3	0.00039	-	7,8-dihydrofolate	recombinant enzyme	Homo sapiens
1.5.1.3	0.001	-	NADPH	for native and recombinant enzymes, monophasic	Homo sapiens
1.5.1.3	0.001	-	NADPH	for native and recombinant enzymes, monophasic	Human gammaherpesvirus 8
1.5.1.3	0.0024	-	7,8-dihydrofolate	native enzyme	Human gammaherpesvirus 8

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.5.1.3	nucleus	wild-type and recombinant viral enzyme in transfected cells	Human gammaherpesvirus 8	5634	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.1.3	Homo sapiens	-	methotrexate-resistant WIL-2 lymphoblastoid cells	-
1.5.1.3	Human gammaherpesvirus 8	-	human virus produced in cultured lymphoma derived B-cells	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.5.1.3	recombinant from Escherichia coli	Human gammaherpesvirus 8

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.1.3	7,8-dihydrofolate + NADPH	-	Homo sapiens	5,6,7,8-tetrahydrofolate + NADP+	-	?
1.5.1.3	7,8-dihydrofolate + NADPH	-	Human gammaherpesvirus 8	5,6,7,8-tetrahydrofolate + NADP+	-	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.5.1.3	7	-	7,8-dihydrofolate	native enzyme	Human gammaherpesvirus 8
1.5.1.3	16.8	-	7,8-dihydrofolate	recombinant enzyme	Homo sapiens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.5.1.3	NADPH	-	Homo sapiens
1.5.1.3	NADPH	-	Human gammaherpesvirus 8

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.5.1.3	additional information	-	additional information	-	Homo sapiens
1.5.1.3	additional information	-	additional information	Ki-values for the native enzyme are higher than for the recombinant enzyme	Human gammaherpesvirus 8
1.5.1.3	0.000000009	-	methotrexate	recombinant enzyme	Homo sapiens
1.5.1.3	0.0000002	-	methotrexate	-	Human gammaherpesvirus 8
1.5.1.3	0.00000061	-	aminopterin	-	Human gammaherpesvirus 8
1.5.1.3	0.000001	-	pyrimethamine	recombinant enzyme	Homo sapiens
1.5.1.3	0.0000039	-	piritrexim	-	Human gammaherpesvirus 8
1.5.1.3	0.000008	-	piritrexim	recombinant enzyme	Homo sapiens
1.5.1.3	0.000029	-	pyrimethamine	-	Human gammaherpesvirus 8
1.5.1.3	0.00081	-	trimethoprim	recombinant enzyme	Homo sapiens
1.5.1.3	0.0023	-	trimethoprim	-	Human gammaherpesvirus 8

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Release 2023.1 (January 2023)