EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | CaCl2 | - |
Lactobacillus leichmannii | |
1.5.1.3 | KBr | - |
Lactobacillus leichmannii | |
1.5.1.3 | KCl | 0.1 M | Lactobacillus leichmannii | |
1.5.1.3 | KI | - |
Lactobacillus leichmannii | |
1.5.1.3 | MgCl2 | - |
Lactobacillus leichmannii | |
1.5.1.3 | Urea | activates at 2 M about 1.5fold | Lactobacillus leichmannii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | deoxytubulosine | - |
Lactobacillus leichmannii | |
1.5.1.3 | methotrexate | competitive | Lactobacillus leichmannii | |
1.5.1.3 | p-chloromercuribenzoate | - |
Lactobacillus leichmannii | |
1.5.1.3 | pegularinine | plant alkaloid | Lactobacillus leichmannii | |
1.5.1.3 | tylophorinidine | plant alkaloid | Lactobacillus leichmannii | |
1.5.1.3 | Urea | at concentration 3-4 M complete inactivation | Lactobacillus leichmannii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0035 | - |
NADPH | - |
Lactobacillus leichmannii | |
1.5.1.3 | 0.0062 | - |
7,8-dihydrofolate | - |
Lactobacillus leichmannii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | CaCl2 | - |
Lactobacillus leichmannii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 20000 | - |
gel filtration | Lactobacillus leichmannii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Lactobacillus leichmannii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | - |
Lactobacillus leichmannii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 200 | - |
purified enzyme | Lactobacillus leichmannii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Lactobacillus leichmannii | 5,6,7,8-tetrahydrofolate + NADP+ | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.3 | monomer | 1 * 20000, SDS-PAGE | Lactobacillus leichmannii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 30 | - |
assay at | Lactobacillus leichmannii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 35 | - |
30% loss of activity after 60 min | Lactobacillus leichmannii |
1.5.1.3 | 45 | - |
90% loss of activity after 30 min | Lactobacillus leichmannii |
1.5.1.3 | 50 | - |
90% loss of activity after 5 min | Lactobacillus leichmannii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 7.4 | - |
- |
Lactobacillus leichmannii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Lactobacillus leichmannii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.000000007 | - |
methotrexate | - |
Lactobacillus leichmannii | |
1.5.1.3 | 0.005 | - |
deoxytubulosine | - |
Lactobacillus leichmannii | |
1.5.1.3 | 0.007 | - |
tylophorinidine | - |
Lactobacillus leichmannii | |
1.5.1.3 | 0.009 | - |
pegularinine | - |
Lactobacillus leichmannii |