Literature summary extracted from

Kaufman, B.T.; Kemerer, V.F.
Purification and characterization of beef liver dihydrofolate reductase (1976), Arch. Biochem. Biophys., 172, 289-300.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.5.1.3	methotrexate	-	Bos taurus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.5.1.3	21300	-	1 * 21300, SDS-PAGE	Bos taurus
1.5.1.3	22500	-	equilibrium sedimentation	Bos taurus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.1.3	Bos taurus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.5.1.3	-	Bos taurus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.5.1.3	liver	-	Bos taurus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.5.1.3	26	-	-	Bos taurus

Storage Stability

EC Number	Storage Stability	Organism
1.5.1.3	-80°C, 1 year	Bos taurus
1.5.1.3	4°C, several weeks	Bos taurus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.1.3	7,8-dihydrofolate + NADPH	-	Bos taurus	5,6,7,8-tetrahydrofolate + NADP+	-	?
1.5.1.3	folate + NADPH	-	Bos taurus	5,6,7,8-tetrahydrofolate + NADP+	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.5.1.3	monomer	1 * 21300, SDS-PAGE	Bos taurus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.5.1.3	28	-	assay at	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)