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Literature summary extracted from

  • Delcamp, T.J.; Susten, S.S.; Blankenship, D.T.; Freisheim, J.H.
    Purification and characterization of dihydrofolate reductase from methotrexate-resistant human lymphoblastoid cells (1983), Biochemistry, 22, 633-639.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.3 KCl
-
Homo sapiens
1.5.1.3 p-chloromercuribenzoate activates in presence of NADPH Homo sapiens
1.5.1.3 p-hydroxymercuribenzoate
-
Homo sapiens
1.5.1.3 Thiourea
-
Homo sapiens
1.5.1.3 Urea
-
Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methylmercuric hydroxide
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
additional information overview Homo sapiens
1.5.1.3 0.036
-
7,8-dihydrofolate
-
Homo sapiens
1.5.1.3 0.25
-
NADPH
-
Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.3 K+ stimulation Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 22000
-
SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Homo sapiens
-
methotrexate-resistant WIL-2 lymphoblastoid cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 HeLa cell
-
Homo sapiens
-
1.5.1.3 WIL-2 cell WIL2 lymphoblastoid cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 16.1
-
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4
-
3 optima: pH 4.0, pH 7.3-7.5 and pH 8.3-8.5 Homo sapiens
1.5.1.3 7.3 7.5 3 optima: pH 4.0, pH 7.3-7.5 and pH 8.3-8.5 Homo sapiens
1.5.1.3 8.3 8.5 3 optima: pH 4.0, pH 7.3-7.5 and pH 8.3-8.5 Homo sapiens