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Literature summary extracted from

  • Morandi, C.; Attardi, G.
    Isolation and characterization of dihydrofolic acid reductase from methotrexate-sensitive and -resistant human cell lines (1981), J. Biol. Chem., 256, 10169-10175.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate cell line HeLa BU-25 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.006 0.007 folate
-
Homo sapiens
1.5.1.3 0.165
-
NADPH
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 22000
-
SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Homo sapiens
-
methotrexate-sensitive cell line HeLa BU-25, methotrexate-insensitive cell line VA2-B
-
1.5.1.3 Homo sapiens
-
methotrexate-resistant WIL-2 lymphoblastoid cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 HeLa cell HeLa cells/BU-25, normal and methotrexate-resistant variant, methotrexate variant of VA2-B cell line Homo sapiens
-
1.5.1.3 WIL-2 cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH enzyme content of methotrexate-insensitive cell line VA2-B is 200fold higher than that of sensitive cell line HeLa BU-25 due to production of alternate enzyme type Homo sapiens 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.3 folate + NADPH
-
Homo sapiens 5,6,7,8-tetrahydrofolate + NADP+
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 1.08
-
folate
-
Homo sapiens
1.5.1.3 8.33
-
7,8-dihydrofolate
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4.8
-
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 4.2 5.6 half-maximal activities at pH 4.2 and pH 5.6 Homo sapiens