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Literature summary extracted from

  • Haenggi, U.J.; Littlefield, J.W.
    Isolation and characterization of the multiple forms of dihydrofolate reductase from methotrexate-resistant hamster cells (1974), J. Biol. Chem., 249, 1390-1397.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.3 Urea activates at 1 M Cricetulus sp.

General Stability

EC Number General Stability Organism
1.5.1.3 bovine serum albumin protects against inactivation Cricetulus sp.
1.5.1.3 unusually resistant to trypsin Cricetulus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate
-
 Cricetulus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.3 K+ stimulation Cricetulus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Cricetulus sp.
-
 2 forms: I, II
-
1.5.1.3 Cricetulus sp.
-
 baby hamster kidney cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 baby hamster kidney cells, 2 forms: I, II Cricetulus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 kidney
-
 Cricetulus sp.
-

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 45
-
 inactivation constant: 0.14 per min form I, 0.13 per min form II Cricetulus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4.2
-
 R-plasmid encoded enzyme, 2 optima, pH 4.2 and pH 7.8, in absence of KCl Cricetulus sp.
1.5.1.3 4.8
-
 in presence of KCl Cricetulus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 additional information
-
 pH-activity profile in absence and presence of KCl Cricetulus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Cricetulus sp.