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Literature summary extracted from

  • Baccanari, D.P.; Tansik, R.L.; Joyner, S.S.; Fling, M.E.; Smith, P.L.
    Characterization of Candida albicans dihydrofolate reductase (1989), J. Biol. Chem., 264, 1100-1107.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Candida albicans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expression in Escherichia coli Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 K+
-
 Candida albicans
1.5.1.3 methotrexate
-
 Candida albicans
1.5.1.3 additional information overview Candida albicans
1.5.1.3 trimethoprim
-
 Candida albicans
1.5.1.3 Urea
-
 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 25000
-
 recombinant from E. coli, gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH Candida albicans key enzyme in biosynthesis of purines, pyrimidines and several amino acids 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 additional information Candida albicans key enzyme in the biosynthesis of purines, pyrimidines and several amino acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 recombinant from Escherichia coli Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 250
-
 purified enzyme Candida albicans

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 4°C, above 1 mg/ml protein concentration, 1 month Candida albicans
1.5.1.3 4°C, pure, 20% glycerol, several weeks Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH key enzyme in biosynthesis of purines, pyrimidines and several amino acids Candida albicans 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 additional information
-
 Candida albicans ?
-
 ?
1.5.1.3 additional information key enzyme in the biosynthesis of purines, pyrimidines and several amino acids Candida albicans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 monomer 1 * 25000, recombinant from Escherichia coli, SDS-PAGE Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 6.3
-
-
 Candida albicans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.00000015
-
 methotrexate recombinant from E. coli Candida albicans
1.5.1.3 0.0043
-
 trimethoprim recombinant from E. coli Candida albicans