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Literature summary extracted from

  • Reddy, V.A.; Rao, N.A.
    Dihydrofolate reductase from soybean seedlings. Characterization of the enzyme purified by affinity chromatography (1976), Arch. Biochem. Biophys., 174, 675-683.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.3 7,8-dihydrofolate protects against heat inactivation Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 5,5'-dithiobis(2-nitrobenzoate)
-
Glycine max
1.5.1.3 aminopterin
-
Glycine max
1.5.1.3 methotrexate
-
Glycine max
1.5.1.3 N-ethylmaleimide
-
Glycine max
1.5.1.3 N10-formylaminopterin
-
Glycine max
1.5.1.3 p-hydroxymercuribenzoate
-
Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.035
-
7,8-dihydrofolate
-
Glycine max
1.5.1.3 0.415
-
NADPH
-
Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 140000
-
gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
Glycine max

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.5.1.3 enzyme denatured with 8 M urea cannot be renatured by removal of urea Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 seedling
-
Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 156.3
-
-
Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
Glycine max 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 More polymer, SDS-PAGE Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 additional information
-
7,8-dihydrofolate protects against heat inactivation Glycine max
1.5.1.3 55
-
5 min, 70% loss of activity, 15 min, more than 80% loss of activity Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 0.95
-
7,8-dihydrofolate
-
Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
Glycine max