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Literature summary extracted from

  • Ratnam, S.; Delcamp, T.J.; Hynes, J.B.; Freisheim, J.H.
    Purification and characterization of dihydrofolate reductase from soybean seedlings (1987), Arch. Biochem. Biophys., 255, 279-289.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.3 freezing and thawing, significant loss of activity even in presence of glycerol Glycine max
1.5.1.3 glycerol, 25%, essential for stabilization during purification Glycine max
1.5.1.3 protease inhibitors phenylmethylsulfonyl fluoride and leupeptin are essential for stabilization during purification Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 2,4-diaminoquinazoline derivatives Glycine max
1.5.1.3 folate
-
 Glycine max
1.5.1.3 K+
-
 Glycine max
1.5.1.3 methotrexate
-
 Glycine max
1.5.1.3 pyrimethamine
-
 Glycine max
1.5.1.3 trimethoprim
-
 Glycine max
1.5.1.3 Urea
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.017
-
 7,8-dihydrofolate
-
 Glycine max
1.5.1.3 0.03
-
 NADPH
-
 Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.3 organic mercurials no activation Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 22000
-
 SDS-PAGE Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 seedling
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 15
-
 crude extract Glycine max
1.5.1.3 56.5
-
 purified enzyme Glycine max

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 4°C, 0.1 M imidazole, pH 7.2, 25% glycerol, stable Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Glycine max 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 ? ? * 22000, SDS-PAGE Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 7.4
-
-
 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Glycine max