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Literature summary extracted from

  • Baccanari, D.; Phillips, A.; Smith, S.; Sinski, D.; Burchall, J.
    Purification and properties of Escherichia coli dihydrofolate reductase (1975), Biochemistry, 14, 5267-5273.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.3 freezing and thawing, stable to repeated freezing and thawing Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 Ba2+
-
 Escherichia coli
1.5.1.3 Ca2+
-
 Escherichia coli
1.5.1.3 Cs+
-
 Escherichia coli
1.5.1.3 Diaminobutane 12 mM Escherichia coli
1.5.1.3 Diaminopentane 12 mM Escherichia coli
1.5.1.3 ethylenediamine 12 mM Escherichia coli
1.5.1.3 K+
-
 Escherichia coli
1.5.1.3 Li+
-
 Escherichia coli
1.5.1.3 Mg2+
-
 Escherichia coli
1.5.1.3 additional information inhibitory effect increased with the radius of the cations Escherichia coli
1.5.1.3 Na+
-
 Escherichia coli
1.5.1.3 Rb+
-
 Escherichia coli
1.5.1.3 spermidine 12 mM Escherichia coli
1.5.1.3 spermine 12 mM Escherichia coli
1.5.1.3 Urea 3 M, 15% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 17300 18300 trimethoprim-resistant, amino acid composition, sedimentation velocity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli
-
 trimethoprim-resistant strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 trimethoprim-resistant Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 19.1
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 4°C for several weeks Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4.5
-
 trimethoprim resistant, 2 optima: pH 4.5 and pH 7.0 Escherichia coli
1.5.1.3 4.5
-
 folic acid reduction Escherichia coli
1.5.1.3 7
-
 trimethoprim resistant, 2 optima: pH 4.5 and pH 7.0 Escherichia coli