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Literature summary extracted from

  • Stone, S.R.; Morrison, J.F.
    Dihydrofolate reductase from Escherichia coli: the kinetic mechanism with NADPH and reduced acetylpyridine adenine dinucleotide phosphate as substrates (1988), Biochemistry, 27, 5493-5499.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ kinetic mechanism Escherichia coli
a
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ rapid random equilibrium mechanism with NADPH and reduced acetylpyridine adenine nucleotide as cofactor Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced
-
 Escherichia coli 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
-
 ?
1.5.1.3 additional information 3-acetylpyridine adenine dinucleotide + dihydrofolate: rapid equilibrium random mechanism Escherichia coli ?
-
 ?
1.5.1.3 additional information NADPH + dihydrofolate: steady-state random mechanism Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 2
-
 7,8-dihydrofolate with acetylpyridine adenine nucleotide Escherichia coli
1.5.1.3 2
-
 acetylpyridine adenine nucleotide with 7,8-dihydrofolate Escherichia coli
1.5.1.3 11
-
 NADPH with 7,8-dihydrofolate Escherichia coli
1.5.1.3 18
-
 7,8-dihydrofolate with NADPH Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 3-acetylpyridine adenine nucleotide
-
 Escherichia coli
1.5.1.3 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 additional information
-
 additional information Ki of substrates dihydrofolate, NADPH and acetylpyridine adenine nucleotide Escherichia coli