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Literature summary extracted from

  • Lei, B.; Tu, S.C.
    Mechanism of reduced flavin transfer from Vibrio harveyi NADPH-FMN oxidoreductase to luciferase (1998), Biochemistry, 37, 14623-14629.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.38 additional information FMN at concentrations over 0.002 mM significantly inhibits the coupled reaction in both light intensity and quantum yield, and shows apparent noncompetitive and competitive inhibition patterns against NADPH and luciferase, respectively. No inhibition of the NADPH oxidation is detected under identical conditions Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.38 additional information
-
 additional information the kinetic mechanism of FRP is changed to a sequential pattern with a Km(FMN) of 0.003 mM and a Km(NADPH) of 0.02 mM in a luciferase-coupled assay measuring light emission Vibrio harveyi
1.5.1.38 0.008
-
 FMN pH 7.0, 23°C, single-enzyme spectrophotometric assay monitoring the NADPH oxidation Vibrio harveyi
1.5.1.38 0.02
-
 NADPH pH 7.0, 23°C, single-enzyme spectrophotometric assay monitoring the NADPH oxidation Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.38 FMN + NADPH + H+ Vibrio harveyi
-
 FMNH2 + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.38 Vibrio harveyi
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.38 FMNH2 + NADP+ = FMN + NADPH + H+ ping-pong kinetic pattern Vibrio harveyi
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.38 FMN + NADPH + H+
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.38 FRP
-
 Vibrio harveyi
1.5.1.38 NADPH-FMN oxidoreductase
-
 Vibrio harveyi