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Literature summary extracted from

  • Yubisui, T.; Matsuki, T.; Takeshita, M.; Yoneyama, Y.
    Characterization of the purified NADPH-flavin reductase of human erythrocytes (1979), J. Biochem., 85, 719-728.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.30 freezing and thawing causes no appreciable loss of the enzyme activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.30 1,10-phenanthroline
-
 Homo sapiens
1.5.1.30 2,3-diphosphoglycerate
-
 Homo sapiens
1.5.1.30 acrinol
-
 Homo sapiens
1.5.1.30 Bathocuproine sulfonate
-
 Homo sapiens
1.5.1.30 Cu2+
-
 Homo sapiens
1.5.1.30 HgCl2
-
 Homo sapiens
1.5.1.30 NADP+ 0.1 mM, about 50% inhibition at no inhibition by NAD+ at pH 7.5, 12% inhibition at pH 4.8 Homo sapiens
1.5.1.30 PCMB
-
 Homo sapiens
1.5.1.30 proflavin hemisulfate
-
 Homo sapiens
1.5.1.30 SeO32-
-
 Homo sapiens
1.5.1.30 Zn2+
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.30 additional information Fe2+, Fe3+, Mg2+, and Ca2+ do not affect the enzyme activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.30 21500
-
 x * 21500, SDS-PAGE Homo sapiens
1.5.1.30 22000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.30
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.30 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.30 0.37
-
 pH 7.0, 25°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.5.1.30 4°C, the purified enzyme is stable at Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FMN + NADPH + H+
-
 Homo sapiens FMNH2 + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.30 ? x * 21500, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 NADPH-FMN reductase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.30 100
-
 boiling of the enzyme leads to complete loss of the FMN reductase activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.30 4.8
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.30 4.5 8 pH 4.5: about 85% of maximal activity, pH 8.0: about 45% of maximal activity Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 NADPH the FMN reductase activity is highly specific for NADPH, and the activity with NADH is 10% of that with NADPH Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.1.30 Homo sapiens
-
-
 8.1