Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gerlo, E.; Charlier, J.
    Identification of NADH-specific and NADPH-specific FMN reductases in Beneckea harveyi (1975), Eur. J. Biochem., 57, 461-467.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.38 0.0005
-
 FMN pH 6.8, 23°C Vibrio harveyi
1.5.1.42 0.0011
-
 FMN pH 6.8, 23°C Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.38 40000
-
 gel filtration Vibrio harveyi
1.5.1.38 63000
-
 sucrose density gradient centrifugation Vibrio harveyi
1.5.1.42 19000
-
 gel filtration Vibrio harveyi
1.5.1.42 31000
-
 sucrose density gradient centrifugation Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.38 FMN + NADPH + H+ Vibrio harveyi
-
 FMNH2 + NADP+
-
 ?
1.5.1.42 FMN + NADH + H+ Vibrio harveyi
-
 FMNH2 + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.38 Vibrio harveyi
-
-
-
1.5.1.42 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.38
-
 Vibrio harveyi
1.5.1.42
-
 Vibrio harveyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.38 1.85
-
 pH 6.8, 23°C Vibrio harveyi
1.5.1.42 2.05
-
 pH 6.8, 23°C Vibrio harveyi

Storage Stability

EC Number Storage Stability Organism
1.5.1.38 -20°C, considerable loss of activity of reductase preparations Vibrio harveyi
1.5.1.38 4°C, considerable loss of activity of reductase preparations Vibrio harveyi
1.5.1.42 -20°C, considerable loss of activity of reductase preparations Vibrio harveyi
1.5.1.42 4°C, considerable loss of activity of reductase preparations Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.38 FMN + NADPH + H+
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+ the affinity of the NADPH-specific reductase for NADPH is 1000 times greater than for NADH Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.42 FMN + NADH + H+
-
 Vibrio harveyi FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+ the affinity of the NADH-specific reductase is about 60 times greater for NADH than for NADPH Vibrio harveyi FMNH2 + NAD+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.38 23
-
 assay at Vibrio harveyi
1.5.1.42 23
-
 assay at Vibrio harveyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.38 55
-
 5 min, complete loss of activity Vibrio harveyi
1.5.1.42 55
-
 5 min, 60% loss of activity Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.38 6.8
-
 assay at Vibrio harveyi
1.5.1.42 6.8
-
 assay at Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.38 NADPH the affinity of the NADPH-specific reductase for NADPH is 1000 times greater than for NADH Vibrio harveyi
1.5.1.42 NADH the affinity of the NADH-specific reductase is about 60 times greater for NADH than for NADPH Vibrio harveyi