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Literature summary extracted from

  • Ruvinov, S.B.; Thompson, J.; Sackett, D.L.; Ginsburg, A.
    Tetrameric N5-(L-1-carboxyethyl)-L-ornithine synthase: Guanidine-HCl-induced unfolding and a low temperature requirement for refolding (1999), Arch. Biochem. Biophys., 371, 115-123.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.24 nutrition industrially important organism used widely as a starter in the dairy industry Lactococcus lactis

General Stability

EC Number General Stability Organism
1.5.1.24 enzymatic activity of CEOS is lost at guanidine-HCl concentrations from 0.5-1.2 M Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.24 34200
-
 4 * 34200, sedimentation equilibrium, 20°C, 3.5 M guanidine-HCl Lactococcus lactis
1.5.1.24 34700
-
 4 * 34700, sedimentation equilibrium, 20°C, 3.0 M guanidine-HCl Lactococcus lactis
1.5.1.24 34900
-
 4 * 34900, sedimentation equilibrium, 20°C Lactococcus lactis
1.5.1.24 35300
-
 4 * 35300 Lactococcus lactis
1.5.1.24 35320
-
 deduced from amino acid sequence Lactococcus lactis
1.5.1.24 38600
-
 4 * 38600, sedimentation equilibrium, 20°C Lactococcus lactis
1.5.1.24 127500
-
 sedimentation equilibrium at 20°C, 3.0 M guanidine-HCl Lactococcus lactis
1.5.1.24 135900
-
 sedimentation equilibrium at 20°C, 3.0 M guanidine-HCl Lactococcus lactis
1.5.1.24 136900
-
 sedimentation equilibrium at 20°C Lactococcus lactis
1.5.1.24 138900
-
 sedimentation equilibrium at 20°C, 3.5 M guanidine-HCl Lactococcus lactis
1.5.1.24 141300
-
 gel filtration, analytical ultracentrifugation, sedimentation equilibrium Lactococcus lactis
1.5.1.24 145700
-
 sedimentation equilibrium at 4°C Lactococcus lactis
1.5.1.24 146700
-
 sedimentation equilibrium at 20°C, 3.5 M guanidine-HCl Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.24 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.24 recombinant enzyme purified from the overproducing strain Escherichia coli CEO571 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.24 L-ornithine + pyruvate + NADPH pyruvic acid serves as an effective keto substrate for the condensation reaction Lactococcus lactis N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ir
1.5.1.24 lysine + pyruvate + NADPH
-
 Lactococcus lactis N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.5.1.24 tetramer 4 * 34900, sedimentation equilibrium, 20°C Lactococcus lactis
1.5.1.24 tetramer 4 * 35300 Lactococcus lactis
1.5.1.24 tetramer 4 * 34200, sedimentation equilibrium, 20°C, 3.5 M guanidine-HCl Lactococcus lactis
1.5.1.24 tetramer 4 * 38600, sedimentation equilibrium, 20°C Lactococcus lactis
1.5.1.24 tetramer 4 * 34700, sedimentation equilibrium, 20°C, 3.0 M guanidine-HCl Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
1.5.1.24 CEOS
-
 Lactococcus lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.24 55
-
 heating CEOS in absence of guanidine-HCl produces irreversible denaturation and loss in activity Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.24 NADPH
-
 Lactococcus lactis