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Literature summary extracted from

  • Payton, C.W.; Chang, Y.F.
    DELTA1-Piperideine-2-carboxylate reductase of Pseudomonas putida (1982), J. Bacteriol., 149, 864-871.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.21 dithiothreitol slight activation at 1 mM Pseudomonas putida
1.5.1.21 EDTA slight activation at 1 mM Pseudomonas putida
1.5.1.21 Sodium diphosphate slight activation at 1 mM Pseudomonas putida

General Stability

EC Number General Stability Organism
1.5.1.21 dithiothreitol, 1 mM, protects during storage Pseudomonas putida
1.5.1.21 EDTA, 1 mM, protects during storage and against heat inactivation at 50°C Pseudomonas putida
1.5.1.21 NADP+, no protection during storage Pseudomonas putida
1.5.1.21 NADPH, protection during storage Pseudomonas putida
1.5.1.21 sodium diphosphate, 1 mM, protects during storage Pseudomonas putida
1.5.1.21 very unstable during purification Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.21 Ca2+ slight inhibition Pseudomonas putida
1.5.1.21 Co2+ almost total inhibition at 0.1 mM Pseudomonas putida
1.5.1.21 Hg2+ almost total inhibition at 0.1 mM Pseudomonas putida
1.5.1.21 KCN slight inhibition Pseudomonas putida
1.5.1.21 Mg2+ slight inhibition Pseudomonas putida
1.5.1.21 Mn2+ almost total inhibition at 0.1 mM Pseudomonas putida
1.5.1.21 additional information no effect: 0.1 mM L-cysteine, 0.1 mM iodoacetate Pseudomonas putida
1.5.1.21 NADPH substrate inhibition above 0.15 mM Pseudomonas putida
1.5.1.21 NaF
-
Pseudomonas putida
1.5.1.21 p-chloromercuribenzoate almost total inhibition at 0.1 mM Pseudomonas putida
1.5.1.21 Zn2+ almost total inhibition at 0.1 mM Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.21 0.13
-
NADPH
-
Pseudomonas putida
1.5.1.21 0.23
-
DELTA1-piperideine-2-carboxylate
-
Pseudomonas putida
1.5.1.21 0.28
-
diglutamate
-
Pseudomonas putida
1.5.1.21 0.4
-
DELTA1-Pyrroline-5-carboxylate
-
Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.21 200000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.21 DELTA1-piperideine-2-carboxylate + NADPH Pseudomonas putida D-lysine metabolism L-pipecolate + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.21 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.21
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.21 14.3
-
-
Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.5.1.21 4°C, total loss of activity of the partially purified enzyme after 8 h, loss of activity can be prevented by adding low concentrations of EDTA, sodium diphosphate, dithiothreitol or L-pipecolic acid Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.21 DELTA1-piperideine-2-carboxylate + NADPH
-
Pseudomonas putida L-pipecolate + NADP+
-
ir
1.5.1.21 DELTA1-piperideine-2-carboxylate + NADPH D-lysine metabolism Pseudomonas putida L-pipecolate + NADP+
-
?
1.5.1.21 DELTA1-pyrroline-5-carboxylate + NADPH + H+ very little activity Pseudomonas putida ?
-
r
1.5.1.21 diglutamate + NADPH + H+ very little activity Pseudomonas putida ?
-
r
1.5.1.21 additional information poor substrates: DELTA1-pyrroline-5-carboxylate, pyroglutamate Pseudomonas putida ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.21 30
-
assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.21 8 8.3
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.21 NADH poor coenzyme Pseudomonas putida
1.5.1.21 NADPH
-
Pseudomonas putida