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Literature summary extracted from

  • Kutzbach, C.; Stokstad, E.L.R.
    Mammalian methylenetetrahydrofolate reductase. Partial purification, properties, and inhibition by S-adenosylmethionine (1971), Biochim. Biophys. Acta, 250, 459-477.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.20 dicoumarol inhibits menadione reductase activity only Sus scrofa
1.5.1.20 Mersalyl 80% inhibition at 1 mM Sus scrofa
1.5.1.20 NADH substrate inhibition above 1 mM Sus scrofa
1.5.1.20 p-chloromercuribenzoate 60% inhibition of the methylenetetrahydrofolate reductase activity at 0.04 mM, 83% inhibition of the menadione reductase activity at 0.04 mM Sus scrofa
1.5.1.20 S-adenosylmethionine allosteric inhibition; inhibition partially reversed by S-adenosylhomocysteine Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.20 0.021
-
 5,10-methylenetetrahydrofolate methylenetetrahydrofolate reductase reaction Sus scrofa
1.5.1.20 0.033
-
 NADPH methylenetetrahydrofolate reductase reaction Sus scrofa
1.5.1.20 0.049
-
 NADPH reduction of menadione Sus scrofa
1.5.1.20 0.085
-
 NADH reduction of menadione Sus scrofa
1.5.1.20 0.31
-
 NADH methylenetetrahydrofolate reductase reaction Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.20 additional information no evidence for participation of heavy metals ions in catalytic process Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH Rattus norvegicus
-
 ?
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH Sus scrofa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.20 Rattus norvegicus
-
-
-
1.5.1.20 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.20
-
 Rattus norvegicus
1.5.1.20
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.20 liver
-
 Rattus norvegicus
-
1.5.1.20 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.20 78
-
-
 Rattus norvegicus
1.5.1.20 153
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + NADH + H+
-
 Sus scrofa 5-methyltetrahydrofolate + NAD+
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH ferricyanide, dichlorophenolindophenol and cytochrome c can act as electron acceptors Sus scrofa 5-methyltetrahydrofolate + NADP+
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH equilibrium far on the side of methyltetrahydrofolate formation Rattus norvegicus 5-methyltetrahydrofolate + NADP+
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH equilibrium far on the side of methyltetrahydrofolate formation Sus scrofa 5-methyltetrahydrofolate + NADP+
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH specific for (+)-diastereoisomer Rattus norvegicus 5-methyltetrahydrofolate + NADP+
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH specific for (+)-diastereoisomer Sus scrofa 5-methyltetrahydrofolate + NADP+
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH
-
 Rattus norvegicus ?
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH
-
 Sus scrofa ?
-
 ?
1.5.1.20 5-methyltetrahydrofolate + oxidized menadione
-
 Rattus norvegicus 5,10-methylenetetrahydrofolate + reduced menadione
-
 ?
1.5.1.20 5-methyltetrahydrofolate + oxidized menadione
-
 Sus scrofa 5,10-methylenetetrahydrofolate + reduced menadione
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.1.20 25 45 methylenetetrahydrofolate reductase reaction, slow increase of activity between 25°C and 45°C Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.20 45
-
 inactivation above Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.20 6.6 6.7 methylenetetrahydrofolate reductase reaction Sus scrofa
1.5.1.20 7.1
-
 menadione reductase reaction Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.20 5.8 8.6 about 60% of activity maximum at pH 5.8, about 50% of activity maximum at pH 8.6, menadione reductase activity Sus scrofa
1.5.1.20 6.2 7.3 half-maximal activity at pH 6.2 and pH 7.3, methylenetetrahydrofolate reductase reaction Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.20 FAD flavoprotein Sus scrofa
1.5.1.20 FMN no effect Rattus norvegicus
1.5.1.20 FMN no effect Sus scrofa
1.5.1.20 NADH low activity Sus scrofa
1.5.1.20 NADPH specific for Sus scrofa