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Literature summary extracted from

  • Rossi, J.R.; Vender, J.; Berg, C.M.; Coleman, W.H.
    Partial purification and some properties of delta1-pyrroline-5-carboxylate reductase from Escherichia coli (1977), J. Bacteriol., 129, 108-114.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 NADH above 0.16 mM Escherichia coli
1.5.1.2 NADP+
-
 Escherichia coli
1.5.1.2 NADPH above 0.13 mM Escherichia coli
1.5.1.2 p-chloromercuribenzoate complete inhibition at 0.125 mM, can be prevented by addition of dithiothreitol Escherichia coli
1.5.1.2 proline
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.14
-
 1-pyrroline-5-carboxylate with NADH Escherichia coli
1.5.1.2 0.15
-
 1-pyrroline-5-carboxylate with NADPH Escherichia coli
1.5.1.2 0.23
-
 NADH NADH Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 320000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 partial Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 41.25
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+ specific for L-isomer Escherichia coli L-proline + NAD(P)+
-
 ir
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Escherichia coli L-proline + NAD+
-
 r
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Escherichia coli L-proline + NADP+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.2 4 40
-
 Escherichia coli
1.5.1.2 60
-
 inactivation above Escherichia coli
1.5.1.2 67
-
 50% inactivation after 5 min Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH preferentially Escherichia coli
1.5.1.2 NADPH preferentially Escherichia coli