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Literature summary extracted from

  • Miler, P.A.; Stewart, C.R.
    Pyrroline-5-carboxylic acid reductase from soybean leaves (1976), Phytochemistry, 15, 1855-1857.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.5.1.2 sensitive to heat and low pH Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 ADP 1 mM Glycine max
1.5.1.2 ATP reversible with Mg2+ Glycine max
1.5.1.2 CTP 91% inactivation at 1 mM reversible with Mg2+ Glycine max
1.5.1.2 GTP 100% inactivation at 10 mM, reversible with Mg2+ Glycine max
1.5.1.2 Hg2+
-
 Glycine max
1.5.1.2 NADP+
-
 Glycine max
1.5.1.2 NH2OH
-
 Glycine max
1.5.1.2 p-chloromercuribenzoate
-
 Glycine max
1.5.1.2 phosphate
-
 Glycine max
1.5.1.2 proline
-
 Glycine max
1.5.1.2 thiazolidine-4-carboxylic acid
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.05
-
 NADPH
-
 Glycine max
1.5.1.2 0.1
-
 NADH
-
 Glycine max
1.5.1.2 0.154
-
 1-pyrroline-5-carboxylate
-
 Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 leaf
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 60
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
1.5.1.2 -10°C, 50% loss of activity in 4 weeks Glycine max
1.5.1.2 4°C, 50% loss of activity in 1 weeks Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Glycine max L-proline + NAD+
-
 r
1.5.1.2 pyrroline-5-carboxylate + NAD(P)H + H+
-
 Glycine max L-proline + NAD(P)+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 7 7.5
-
 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH preferentially Glycine max
1.5.1.2 NADPH
-
 Glycine max