Any feedback?
Literature summary extracted from
- Purification and characterization of pyrroline-5-carboxylate reductase from bovine retina (1982), Biochim. Biophys. Acta, 717, 215-219.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.2 | ATP | competitive with respect to NADH, 50% inhibition at 0.1 mM | Bos taurus |  |
| 1.5.1.2 | proline | 39% inhibition of the NADH dependent activity and 17% of the NADPH dependent activity at 10 mM | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.2 | 0.0071 | - |
NADPH | - |
Bos taurus | |
| 1.5.1.2 | 0.125 | - |
1-pyrroline-5-carboxylate | - |
Bos taurus | |
| 1.5.1.2 | 0.2 | - |
NADH | - |
Bos taurus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.2 | KCl | 2fold increase at 70 mM | Bos taurus | |
| 1.5.1.2 | phosphate | 10 mM, reaction enhancement | Bos taurus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.2 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.2 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.1.2 | retina | distribution in bovine cornea and lens | Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.2 | additional information | - |
subcellular distribution | Bos taurus |
| 1.5.1.2 | 4.167 | - |
- |
Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.5.1.2 | -20°C, inactivation in few days, unstable in the absence of NADPH | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.2 | 1-pyrroline-5-carboxylate + NADH + H+ | - |
Bos taurus | L-proline + NAD+ | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.2 | 7 | 7.5 | - |
Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.2 | NADH | - |
Bos taurus | |
| 1.5.1.2 | NADPH | - |
Bos taurus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.2 | 0.1 | - |
ATP | - |
Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
