Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Otten, L.A.B.M.; Vreugdenhil, D.; Schilperoort, R.A.
    Properties of D(+)-lysopine dehydrogenase from crown gall tumour tissue (1977), Biochim. Biophys. Acta, 485, 268-277.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.16 0.003
-
 NADPH
-
 Nicotiana tabacum
1.5.1.16 0.01
-
 NADP+
-
 Nicotiana tabacum
1.5.1.16 0.16
-
 D(+)-lysopine
-
 Nicotiana tabacum
1.5.1.16 0.23
-
 pyruvate
-
 Nicotiana tabacum
1.5.1.16 0.56
-
 L-lysine
-
 Nicotiana tabacum
1.5.1.16 0.8
-
 D-(+)-octopine
-
 Nicotiana tabacum
1.5.1.16 1.3
-
 L-methionine
-
 Nicotiana tabacum
1.5.1.16 2.3
-
 L-ornithine
-
 Nicotiana tabacum
1.5.1.16 4.4
-
 L-arginine
-
 Nicotiana tabacum
1.5.1.16 6.1
-
 L-glutamine
-
 Nicotiana tabacum
1.5.1.16 6.1
-
 L-histidine
-
 Nicotiana tabacum
1.5.1.16 17.1
-
 L-citrulline
-
 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.16 N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O Nicotiana tabacum
-
 L-lysine + pyruvate + NADPH
-
 r
1.5.1.16 N2-(D-1-carboxyethyl)-L-ornithine + NADP+ + H2O Nicotiana tabacum
-
 L-ornithine + pyruvate + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.16 Nicotiana tabacum
-
 leaf tumors initiated by infection with Agrobacterium tumefaciens, strain B6S3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.16 leaf tumors initiated by infection with Agrobacterium tumefaciens, partial Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.16 crown gall leaves infected with Agrobacterium tumefaciens strain B6S3 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.16 1.045
-
-
 Nicotiana tabacum

Storage Stability

EC Number Storage Stability Organism
1.5.1.16 -20°C, many weeks Nicotiana tabacum
1.5.1.16 4°C, some days Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.16 D-(+)-octopine + pyruvate + NADH + H+
-
 Nicotiana tabacum ?
-
 r
1.5.1.16 L-arginine + pyruvate + NADH + H+
-
 Nicotiana tabacum ?
-
 r
1.5.1.16 L-citrulline + pyruvate + NADH + H+
-
 Nicotiana tabacum ?
-
 r
1.5.1.16 L-glutamine + pyruvate + NADH + H+
-
 Nicotiana tabacum ?
-
 r
1.5.1.16 L-histidine + pyruvate + NADH + H+
-
 Nicotiana tabacum ?
-
 r
1.5.1.16 L-methionine + pyruvate + NADH + H+
-
 Nicotiana tabacum ?
-
 r
1.5.1.16 N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
-
 Nicotiana tabacum L-lysine + pyruvate + NADPH
-
 r
1.5.1.16 N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O N2-(D-1-carboxyethyl)-L-lysine is identical with lysopine, in the reverse reaction L-methionine, L-ornithine, L-arginine, L-histidine, L-glutamine can act instead of L-lysine Nicotiana tabacum L-lysine + pyruvate + NADPH
-
 r
1.5.1.16 N2-(D-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 Nicotiana tabacum L-ornithine + pyruvate + NADPH
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.16 30
-
 assay at Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.16 6.2
-
 L-arginine Nicotiana tabacum
1.5.1.16 6.3
-
 L-methionine Nicotiana tabacum
1.5.1.16 6.7
-
 L-ornithine Nicotiana tabacum
1.5.1.16 8.9
-
 lysopine Nicotiana tabacum
1.5.1.16 9.1
-
 N2-(D-1-carboxyethyl)-L-ornithine i.e. octopine Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.16 NADH
-
 Nicotiana tabacum
1.5.1.16 NADPH
-
 Nicotiana tabacum