BRENDA - Enzyme Database show

Enzymes of DELTA1-pyrroline-5-carboxylate metabolism in the camel tick Hyalomma dromedarii during embryogenesis. Purification and characterization of DELTA1-pyrroline-5-carboxylate dehydrogenases

Fahmy, A.S.; Mohamed, S.A.; Girgis, R.B.; Abdel-Ghaffar, F.A.; Comp. Biochem. Physiol. B 118, 229-237 (1997)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.88
4-aminobutyrate
both isoforms
Hyalomma dromedarii
1.2.1.88
ADP
3 mM, 14% inhibition of isoform A and 16% inhibition of isoform B
Hyalomma dromedarii
1.2.1.88
AMP
3 mM, 33% inhibition of isoform A and 11% inhibition of isoform B
Hyalomma dromedarii
1.2.1.88
arsenite
both isoforms
Hyalomma dromedarii
1.2.1.88
ATP
inhibits isoform B by 33%, but not isoform A
Hyalomma dromedarii
1.2.1.88
Co2+
both isoforms, concentration above 3 mM
Hyalomma dromedarii
1.2.1.88
Gly
-
Hyalomma dromedarii
1.2.1.88
Hg2+
both isoforms
Hyalomma dromedarii
1.2.1.88
iodoacetamide
both isoforms
Hyalomma dromedarii
1.2.1.88
L-Ala
-
Hyalomma dromedarii
1.2.1.88
L-Glu
-
Hyalomma dromedarii
1.2.1.88
L-hydroxyproline
both isoforms, potent inhibition
Hyalomma dromedarii
1.2.1.88
L-Leu
-
Hyalomma dromedarii
1.2.1.88
L-Ser
-
Hyalomma dromedarii
1.2.1.88
L-Val
-
Hyalomma dromedarii
1.2.1.88
Ni2+
both isoforms
Hyalomma dromedarii
1.2.1.88
p-hydroxymercuribenzoate
both isoforms, potent inhibition
Hyalomma dromedarii
1.2.1.88
proline
both isoforms
Hyalomma dromedarii
1.2.1.88
Zn2+
both isoforms
Hyalomma dromedarii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.88
0.133
-
D-glucose
isoform A
Hyalomma dromedarii
1.2.1.88
0.2
-
1-pyrroline-5-carboxylate
isoform B, with NADP+
Hyalomma dromedarii
1.2.1.88
0.2
-
indol-3-acetaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.25
-
4-methoxybenzaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.27
-
p-nitrobenzaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.3
-
1-pyrroline-5-carboxylate
isoform A, with NADP+; isoform B, with NAD+
Hyalomma dromedarii
1.2.1.88
0.33
-
1-pyrroline-5-carboxylate
isoform A, with NAD+
Hyalomma dromedarii
1.2.1.88
0.5
-
3-Methoxybenzaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.5
-
benzaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.62
-
NAD+
isoform A
Hyalomma dromedarii
1.2.1.88
0.76
-
formaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
1
-
acetaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
1
-
glyoxylic acid
isoform A
Hyalomma dromedarii
1.2.1.88
1
-
NADP+
isoform A
Hyalomma dromedarii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.88
50000
-
isoform B, gel filtration
Hyalomma dromedarii
1.2.1.88
52000
-
1 * 52000, isoform B, SDS-PAGE
Hyalomma dromedarii
1.2.1.88
59000
-
1 * 59000, isoform A, SDS-PAGE
Hyalomma dromedarii
1.2.1.88
60000
-
isoform A, gel filtration
Hyalomma dromedarii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.88
Hyalomma dromedarii
-
camel tick; two isoforms A and B
-
1.5.1.2
Hyalomma dromedarii
-
camel
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.88
amino acid composition determination
Hyalomma dromedarii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.88
embryo
-
Hyalomma dromedarii
-
1.2.1.88
larva
-
Hyalomma dromedarii
-
1.5.1.2
embryo
-
Hyalomma dromedarii
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.88
0.151
-
embryo, 24 days after oviposition
Hyalomma dromedarii
1.2.1.88
0.223
-
larvae
Hyalomma dromedarii
1.2.1.88
9.75
-
purified enzyme
Hyalomma dromedarii
1.5.1.2
additional information
-
specific activity in different developmental stages of the embryo
Hyalomma dromedarii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.88
1-pyrroline-5-carboxylate + NAD+ + H2O
-
392065
Hyalomma dromedarii
L-glutamate + NADH
-
-
-
?
1.2.1.88
3-methoxybenzaldehyde + NAD+ + H2O
as active as 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B
392065
Hyalomma dromedarii
3-methoxybenzoate + NADH
-
-
-
?
1.2.1.88
4-methoxybenzaldehyde + NAD+ + H2O
as active as 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B
392065
Hyalomma dromedarii
4-methoxybenzoate + NADH + H+
-
-
-
?
1.2.1.88
4-nitrobenzaldehyde + NAD+ + H2O
as active as 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
4-nitrobenzoate + NADH + H+
-
-
-
?
1.2.1.88
acetaldehyde + NAD+ + H2O
41% activity compared to 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
acetate + NADH + H+
-
-
-
?
1.2.1.88
benzaldehyde + NAD+ + H2O
2fold higher activity than with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
benzoate + NADH + H+
-
-
-
?
1.2.1.88
D-glucose + NAD+ + H2O
80% of the activity with 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B
392065
Hyalomma dromedarii
D-gluconate + NADH
-
-
-
?
1.2.1.88
formaldehyde + NAD+ + H2O
50% of activity with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
formate + NADH
-
-
-
?
1.2.1.88
glyceralaldehyde-3-phosphate + NAD+ + H2O
slight activity with isoforms A and B
392065
Hyalomma dromedarii
glycerate-3-phosphate + NADH
-
-
-
?
1.2.1.88
glyoxylic acid + NAD+ + H2O
41% activity of that with 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B
392065
Hyalomma dromedarii
oxalic acid + NADH
-
-
-
?
1.2.1.88
indole-3-acetaldehyde + NAD+ + H2O
66% activity of that with 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
indole-3-acetate + NADH + H+
-
-
-
?
1.2.1.88
indole-3-phosphate + NAD+ + H2O
slight activity with isoforms A and B
392065
Hyalomma dromedarii
?
-
-
-
?
1.2.1.88
additional information
additional esterase activity with p-nitrophenyl acetate as substrate, 50% of activity with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.88
monomer
1 * 52000, isoform B, SDS-PAGE; 1 * 59000, isoform A, SDS-PAGE
Hyalomma dromedarii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.88
7.5
-
both isoforms
Hyalomma dromedarii
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.88
2.5
-
proline
isoform B
Hyalomma dromedarii
1.2.1.88
3
-
gamma-aminobutyrate
isoform B
Hyalomma dromedarii
1.2.1.88
3
-
proline
isoform A
Hyalomma dromedarii
1.2.1.88
3.4
-
glutamate
isoform B
Hyalomma dromedarii
1.2.1.88
3.75
-
gamma-aminobutyrate
isoform A
Hyalomma dromedarii
1.2.1.88
3.75
-
hydroxyproline
isoform B
Hyalomma dromedarii
1.2.1.88
4.75
-
hydroxyproline
isoform A
Hyalomma dromedarii
1.2.1.88
7
-
glutamate
isoform A
Hyalomma dromedarii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.88
4-aminobutyrate
both isoforms
Hyalomma dromedarii
1.2.1.88
ADP
3 mM, 14% inhibition of isoform A and 16% inhibition of isoform B
Hyalomma dromedarii
1.2.1.88
AMP
3 mM, 33% inhibition of isoform A and 11% inhibition of isoform B
Hyalomma dromedarii
1.2.1.88
arsenite
both isoforms
Hyalomma dromedarii
1.2.1.88
ATP
inhibits isoform B by 33%, but not isoform A
Hyalomma dromedarii
1.2.1.88
Co2+
both isoforms, concentration above 3 mM
Hyalomma dromedarii
1.2.1.88
Gly
-
Hyalomma dromedarii
1.2.1.88
Hg2+
both isoforms
Hyalomma dromedarii
1.2.1.88
iodoacetamide
both isoforms
Hyalomma dromedarii
1.2.1.88
L-Ala
-
Hyalomma dromedarii
1.2.1.88
L-Glu
-
Hyalomma dromedarii
1.2.1.88
L-hydroxyproline
both isoforms, potent inhibition
Hyalomma dromedarii
1.2.1.88
L-Leu
-
Hyalomma dromedarii
1.2.1.88
L-Ser
-
Hyalomma dromedarii
1.2.1.88
L-Val
-
Hyalomma dromedarii
1.2.1.88
Ni2+
both isoforms
Hyalomma dromedarii
1.2.1.88
p-hydroxymercuribenzoate
both isoforms, potent inhibition
Hyalomma dromedarii
1.2.1.88
proline
both isoforms
Hyalomma dromedarii
1.2.1.88
Zn2+
both isoforms
Hyalomma dromedarii
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.88
2.5
-
proline
isoform B
Hyalomma dromedarii
1.2.1.88
3
-
gamma-aminobutyrate
isoform B
Hyalomma dromedarii
1.2.1.88
3
-
proline
isoform A
Hyalomma dromedarii
1.2.1.88
3.4
-
glutamate
isoform B
Hyalomma dromedarii
1.2.1.88
3.75
-
gamma-aminobutyrate
isoform A
Hyalomma dromedarii
1.2.1.88
3.75
-
hydroxyproline
isoform B
Hyalomma dromedarii
1.2.1.88
4.75
-
hydroxyproline
isoform A
Hyalomma dromedarii
1.2.1.88
7
-
glutamate
isoform A
Hyalomma dromedarii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.88
0.133
-
D-glucose
isoform A
Hyalomma dromedarii
1.2.1.88
0.2
-
1-pyrroline-5-carboxylate
isoform B, with NADP+
Hyalomma dromedarii
1.2.1.88
0.2
-
indol-3-acetaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.25
-
4-methoxybenzaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.27
-
p-nitrobenzaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.3
-
1-pyrroline-5-carboxylate
isoform A, with NADP+; isoform B, with NAD+
Hyalomma dromedarii
1.2.1.88
0.33
-
1-pyrroline-5-carboxylate
isoform A, with NAD+
Hyalomma dromedarii
1.2.1.88
0.5
-
3-Methoxybenzaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.5
-
benzaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
0.62
-
NAD+
isoform A
Hyalomma dromedarii
1.2.1.88
0.76
-
formaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
1
-
acetaldehyde
isoform A
Hyalomma dromedarii
1.2.1.88
1
-
glyoxylic acid
isoform A
Hyalomma dromedarii
1.2.1.88
1
-
NADP+
isoform A
Hyalomma dromedarii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.88
50000
-
isoform B, gel filtration
Hyalomma dromedarii
1.2.1.88
52000
-
1 * 52000, isoform B, SDS-PAGE
Hyalomma dromedarii
1.2.1.88
59000
-
1 * 59000, isoform A, SDS-PAGE
Hyalomma dromedarii
1.2.1.88
60000
-
isoform A, gel filtration
Hyalomma dromedarii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.88
amino acid composition determination
Hyalomma dromedarii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.88
embryo
-
Hyalomma dromedarii
-
1.2.1.88
larva
-
Hyalomma dromedarii
-
1.5.1.2
embryo
-
Hyalomma dromedarii
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.88
0.151
-
embryo, 24 days after oviposition
Hyalomma dromedarii
1.2.1.88
0.223
-
larvae
Hyalomma dromedarii
1.2.1.88
9.75
-
purified enzyme
Hyalomma dromedarii
1.5.1.2
additional information
-
specific activity in different developmental stages of the embryo
Hyalomma dromedarii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.88
1-pyrroline-5-carboxylate + NAD+ + H2O
-
392065
Hyalomma dromedarii
L-glutamate + NADH
-
-
-
?
1.2.1.88
3-methoxybenzaldehyde + NAD+ + H2O
as active as 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B
392065
Hyalomma dromedarii
3-methoxybenzoate + NADH
-
-
-
?
1.2.1.88
4-methoxybenzaldehyde + NAD+ + H2O
as active as 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B
392065
Hyalomma dromedarii
4-methoxybenzoate + NADH + H+
-
-
-
?
1.2.1.88
4-nitrobenzaldehyde + NAD+ + H2O
as active as 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
4-nitrobenzoate + NADH + H+
-
-
-
?
1.2.1.88
acetaldehyde + NAD+ + H2O
41% activity compared to 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
acetate + NADH + H+
-
-
-
?
1.2.1.88
benzaldehyde + NAD+ + H2O
2fold higher activity than with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
benzoate + NADH + H+
-
-
-
?
1.2.1.88
D-glucose + NAD+ + H2O
80% of the activity with 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B
392065
Hyalomma dromedarii
D-gluconate + NADH
-
-
-
?
1.2.1.88
formaldehyde + NAD+ + H2O
50% of activity with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
formate + NADH
-
-
-
?
1.2.1.88
glyceralaldehyde-3-phosphate + NAD+ + H2O
slight activity with isoforms A and B
392065
Hyalomma dromedarii
glycerate-3-phosphate + NADH
-
-
-
?
1.2.1.88
glyoxylic acid + NAD+ + H2O
41% activity of that with 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B
392065
Hyalomma dromedarii
oxalic acid + NADH
-
-
-
?
1.2.1.88
indole-3-acetaldehyde + NAD+ + H2O
66% activity of that with 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
indole-3-acetate + NADH + H+
-
-
-
?
1.2.1.88
indole-3-phosphate + NAD+ + H2O
slight activity with isoforms A and B
392065
Hyalomma dromedarii
?
-
-
-
?
1.2.1.88
additional information
additional esterase activity with p-nitrophenyl acetate as substrate, 50% of activity with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B
392065
Hyalomma dromedarii
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.88
monomer
1 * 52000, isoform B, SDS-PAGE; 1 * 59000, isoform A, SDS-PAGE
Hyalomma dromedarii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.88
7.5
-
both isoforms
Hyalomma dromedarii