BRENDA - Enzyme Database show

DELTA1-pyrroline-5-carboxylate dehydrogenase (ox liver) (oxidation of DELTA1-pyrroline-5-carboxylate to glutamate)

Strecker, H.J.; Methods Enzymol. 17B, 262-265 (1971)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.88
4-aminobutyrate
-
Bos taurus
1.2.1.88
5-Aminopentanoate
-
Bos taurus
1.2.1.88
6-aminohexanoate
-
Bos taurus
1.2.1.88
ADP
-
Bos taurus
1.2.1.88
AMP
-
Bos taurus
1.2.1.88
arsenite
-
Bos taurus
1.2.1.88
ATP
-
Bos taurus
1.2.1.88
L-hydroxyproline
-
Bos taurus
1.2.1.88
p-hydroxymercuribenzoate
-
Bos taurus
1.2.1.88
piperidin-2-one
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.88
0.053
-
NAD+
-
Bos taurus
1.2.1.88
0.062
-
1-pyrroline-5-carboxylate
with NADP+
Bos taurus
1.2.1.88
0.12
-
NADP+
-
Bos taurus
1.2.1.88
0.29
-
1-pyrroline-5-carboxylate
with NAD+
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.88
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.88
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.88
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.88
0.55
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.88
-15°C, 50% loss of activity, 2 months
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.88
1-pyrroline-5-carboxylate + NAD+ + H2O
-
392053
Bos taurus
L-glutamate + NADH
-
392053
Bos taurus
ir
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.88
8.5
8.6
-
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.88
NAD+
preferentially
Bos taurus
1.2.1.88
NADP+
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.88
NAD+
preferentially
Bos taurus
1.2.1.88
NADP+
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.88
4-aminobutyrate
-
Bos taurus
1.2.1.88
5-Aminopentanoate
-
Bos taurus
1.2.1.88
6-aminohexanoate
-
Bos taurus
1.2.1.88
ADP
-
Bos taurus
1.2.1.88
AMP
-
Bos taurus
1.2.1.88
arsenite
-
Bos taurus
1.2.1.88
ATP
-
Bos taurus
1.2.1.88
L-hydroxyproline
-
Bos taurus
1.2.1.88
p-hydroxymercuribenzoate
-
Bos taurus
1.2.1.88
piperidin-2-one
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.88
0.053
-
NAD+
-
Bos taurus
1.2.1.88
0.062
-
1-pyrroline-5-carboxylate
with NADP+
Bos taurus
1.2.1.88
0.12
-
NADP+
-
Bos taurus
1.2.1.88
0.29
-
1-pyrroline-5-carboxylate
with NAD+
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.88
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.88
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.88
0.55
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.88
-15°C, 50% loss of activity, 2 months
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.88
1-pyrroline-5-carboxylate + NAD+ + H2O
-
392053
Bos taurus
L-glutamate + NADH
-
392053
Bos taurus
ir
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.88
8.5
8.6
-
Bos taurus