BRENDA - Enzyme Database

Enzymatic synthesis of L-pipecolic acid and L-proline

Meister, A.; Radhakrishnan, A.N.; Buckley, S.D.; J. Biol. Chem. 229, 789-800 (1957)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.1
0.038
-
NADPH
+ DELTA1-pyrroline-2-carboxylate
Rattus norvegicus
1.5.1.1
0.043
-
NADH
+ DELTA1-pyrroline-2-carboxylate
Rattus norvegicus
1.5.1.1
0.06
-
DELTA1-piperidine-2-carboxylate
cosubstrate NADH
Rattus norvegicus
1.5.1.1
0.074
-
NADH
+ DELTA1-piperidine-2-carboxylate
Rattus norvegicus
1.5.1.1
0.082
-
DELTA1-pyrroline-2-carboxylate
cosubstrate NADH
Rattus norvegicus
1.5.1.1
0.12
-
DELTA1-pyrroline-2-carboxylate
cosubstrate NADPH
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Vigna radiata var. radiata
-
L-pipecolic acid + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Rattus norvegicus
-
L-pipecolic acid + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Neurospora crassa
-
L-pipecolic acid + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Pisum sativum
-
L-pipecolic acid + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Klebsiella aerogenes
-
L-pipecolic acid + NAD(P)+
-
-
-
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Vigna radiata var. radiata
-
L-proline + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Rattus norvegicus
-
L-proline + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Neurospora crassa
-
L-proline + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Pisum sativum
-
L-proline + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Klebsiella aerogenes
-
L-proline + NAD(P)+
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.5.1.1
Klebsiella aerogenes
-
-
-
1.5.1.1
Neurospora crassa
-
-
-
1.5.1.1
Pisum sativum
-
-
-
1.5.1.1
Rattus norvegicus
-
-
-
1.5.1.1
Vigna radiata var. radiata
-
-
-
1.5.1.2
Pisum sativum
-
-
-
1.5.1.2
Rattus norvegicus
-
-
-
1.5.1.2
Vigna radiata var. radiata
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.5.1.1
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.1
brain
-
Rattus norvegicus
-
1.5.1.1
heart
-
Rattus norvegicus
-
1.5.1.1
kidney
-
Rattus norvegicus
-
1.5.1.1
liver
-
Rattus norvegicus
-
1.5.1.1
seed
germinated seed
Pisum sativum
-
1.5.1.1
seed
germinated seed
Vigna radiata var. radiata
-
1.5.1.1
skeletal muscle
-
Rattus norvegicus
-
1.5.1.1
spleen
-
Rattus norvegicus
-
1.5.1.1
testis
-
Rattus norvegicus
-
1.5.1.2
kidney
-
Rattus norvegicus
-
1.5.1.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.5.1.1
additional information
-
specific activities in homogenates of various organs
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Vigna radiata var. radiata
L-pipecolic acid + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Vigna radiata var. radiata
L-pipecolic acid + NAD(P)+
-
392038
Vigna radiata var. radiata
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Rattus norvegicus
L-pipecolic acid + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Rattus norvegicus
L-pipecolic acid + NAD(P)+
-
392038
Rattus norvegicus
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Neurospora crassa
L-pipecolic acid + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Neurospora crassa
L-pipecolic acid + NAD(P)+
-
392038
Neurospora crassa
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Pisum sativum
L-pipecolic acid + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Pisum sativum
L-pipecolic acid + NAD(P)+
-
392038
Pisum sativum
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Klebsiella aerogenes
L-pipecolic acid + NAD(P)+
-
-
-
-
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Vigna radiata var. radiata
L-proline + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Vigna radiata var. radiata
L-proline + NAD(P)+
-
392038
Vigna radiata var. radiata
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Rattus norvegicus
L-proline + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Rattus norvegicus
L-proline + NAD(P)+
-
392038
Rattus norvegicus
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Neurospora crassa
L-proline + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Neurospora crassa
L-proline + NAD(P)+
-
392038
Neurospora crassa
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Pisum sativum
L-proline + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Pisum sativum
L-proline + NAD(P)+
-
392038
Pisum sativum
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Klebsiella aerogenes
L-proline + NAD(P)+
-
-
-
-
1.5.1.2
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
392038
Vigna radiata var. radiata
L-proline + NAD(P)+
-
-
-
?
1.5.1.2
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
392038
Rattus norvegicus
L-proline + NAD(P)+
-
-
-
-
1.5.1.2
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
392038
Pisum sativum
L-proline + NAD(P)+
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.1
6
-
DELTA1-piperidine-2-carboxylate
Rattus norvegicus
1.5.1.1
6.8
-
DELTA1-pyrroline-2-carboxylate
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.1
NADH
-
Rattus norvegicus
1.5.1.1
NADH
-
Klebsiella aerogenes
1.5.1.1
NADH
-
Neurospora crassa
1.5.1.1
NADH
-
Pisum sativum
1.5.1.1
NADH
-
Vigna radiata var. radiata
1.5.1.1
NADPH
-
Rattus norvegicus
1.5.1.1
NADPH
-
Klebsiella aerogenes
1.5.1.1
NADPH
-
Neurospora crassa
1.5.1.1
NADPH
-
Pisum sativum
1.5.1.1
NADPH
-
Vigna radiata var. radiata
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.1
NADH
-
Rattus norvegicus
1.5.1.1
NADH
-
Klebsiella aerogenes
1.5.1.1
NADH
-
Neurospora crassa
1.5.1.1
NADH
-
Pisum sativum
1.5.1.1
NADH
-
Vigna radiata var. radiata
1.5.1.1
NADPH
-
Rattus norvegicus
1.5.1.1
NADPH
-
Klebsiella aerogenes
1.5.1.1
NADPH
-
Neurospora crassa
1.5.1.1
NADPH
-
Pisum sativum
1.5.1.1
NADPH
-
Vigna radiata var. radiata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.1
0.038
-
NADPH
+ DELTA1-pyrroline-2-carboxylate
Rattus norvegicus
1.5.1.1
0.043
-
NADH
+ DELTA1-pyrroline-2-carboxylate
Rattus norvegicus
1.5.1.1
0.06
-
DELTA1-piperidine-2-carboxylate
cosubstrate NADH
Rattus norvegicus
1.5.1.1
0.074
-
NADH
+ DELTA1-piperidine-2-carboxylate
Rattus norvegicus
1.5.1.1
0.082
-
DELTA1-pyrroline-2-carboxylate
cosubstrate NADH
Rattus norvegicus
1.5.1.1
0.12
-
DELTA1-pyrroline-2-carboxylate
cosubstrate NADPH
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Vigna radiata var. radiata
-
L-pipecolic acid + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Rattus norvegicus
-
L-pipecolic acid + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Neurospora crassa
-
L-pipecolic acid + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Pisum sativum
-
L-pipecolic acid + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
Klebsiella aerogenes
-
L-pipecolic acid + NAD(P)+
-
-
-
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Vigna radiata var. radiata
-
L-proline + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Rattus norvegicus
-
L-proline + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Neurospora crassa
-
L-proline + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Pisum sativum
-
L-proline + NAD(P)+
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
Klebsiella aerogenes
-
L-proline + NAD(P)+
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.1
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.1
brain
-
Rattus norvegicus
-
1.5.1.1
heart
-
Rattus norvegicus
-
1.5.1.1
kidney
-
Rattus norvegicus
-
1.5.1.1
liver
-
Rattus norvegicus
-
1.5.1.1
seed
germinated seed
Pisum sativum
-
1.5.1.1
seed
germinated seed
Vigna radiata var. radiata
-
1.5.1.1
skeletal muscle
-
Rattus norvegicus
-
1.5.1.1
spleen
-
Rattus norvegicus
-
1.5.1.1
testis
-
Rattus norvegicus
-
1.5.1.2
kidney
-
Rattus norvegicus
-
1.5.1.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.5.1.1
additional information
-
specific activities in homogenates of various organs
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Vigna radiata var. radiata
L-pipecolic acid + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Vigna radiata var. radiata
L-pipecolic acid + NAD(P)+
-
392038
Vigna radiata var. radiata
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Rattus norvegicus
L-pipecolic acid + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Rattus norvegicus
L-pipecolic acid + NAD(P)+
-
392038
Rattus norvegicus
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Neurospora crassa
L-pipecolic acid + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Neurospora crassa
L-pipecolic acid + NAD(P)+
-
392038
Neurospora crassa
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Pisum sativum
L-pipecolic acid + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Pisum sativum
L-pipecolic acid + NAD(P)+
-
392038
Pisum sativum
ir
1.5.1.1
DELTA1-piperidine-2-carboxylate + NAD(P)H
-
392038
Klebsiella aerogenes
L-pipecolic acid + NAD(P)+
-
-
-
-
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Vigna radiata var. radiata
L-proline + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Vigna radiata var. radiata
L-proline + NAD(P)+
-
392038
Vigna radiata var. radiata
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Rattus norvegicus
L-proline + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Rattus norvegicus
L-proline + NAD(P)+
-
392038
Rattus norvegicus
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Neurospora crassa
L-proline + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Neurospora crassa
L-proline + NAD(P)+
-
392038
Neurospora crassa
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Pisum sativum
L-proline + NAD(P)+
-
-
-
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Pisum sativum
L-proline + NAD(P)+
-
392038
Pisum sativum
ir
1.5.1.1
DELTA1-pyrroline-2-carboxylate + NAD(P)H
-
392038
Klebsiella aerogenes
L-proline + NAD(P)+
-
-
-
-
1.5.1.2
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
392038
Vigna radiata var. radiata
L-proline + NAD(P)+
-
-
-
?
1.5.1.2
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
392038
Rattus norvegicus
L-proline + NAD(P)+
-
-
-
-
1.5.1.2
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
392038
Pisum sativum
L-proline + NAD(P)+
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.1
6
-
DELTA1-piperidine-2-carboxylate
Rattus norvegicus
1.5.1.1
6.8
-
DELTA1-pyrroline-2-carboxylate
Rattus norvegicus