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Literature summary extracted from

  • Cullimore, J.V.; Sims, A.P.
    Occurrence of two forms of glutamate synthase in Chlamydomonas reinhardii (1981), Phytochemistry, 20, 597-600.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.4.1.14 2-mercaptoethanol stabilizes Chlamydomonas reinhardtii
1.4.1.14 high levels of sucrose stabilize Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.14 azaserine
-
 Chlamydomonas reinhardtii
1.4.7.1 azaserine
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.14 0.007
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii
1.4.1.14 0.013
-
 NADH
-
 Chlamydomonas reinhardtii
1.4.1.14 0.9
-
 L-glutamine
-
 Chlamydomonas reinhardtii
1.4.7.1 0.17
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii
1.4.7.1 0.19
-
 L-glutamine
-
 Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.14 240000
-
 sedimentation studies Chlamydomonas reinhardtii
1.4.7.1 165000
-
 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Chlamydomonas reinhardtii ammonia assimilation glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Chlamydomonas reinhardtii CCAP 11/32A ammonia assimilation glutamate + NAD+
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin Chlamydomonas reinhardtii
-
 L-glutamate + oxidized ferredoxin
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin Chlamydomonas reinhardtii CCAP 11/32A
-
 L-glutamate + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.14 Chlamydomonas reinhardtii
-
-
-
1.4.1.14 Chlamydomonas reinhardtii CCAP 11/32A
-
-
-
1.4.7.1 Chlamydomonas reinhardtii
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.4.1.14 sensitive to oxygen Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.14 partial purification Chlamydomonas reinhardtii
1.4.7.1 partially purified Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Chlamydomonas reinhardtii L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Chlamydomonas reinhardtii CCAP 11/32A L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH ammonia assimilation Chlamydomonas reinhardtii glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH ammonia assimilation Chlamydomonas reinhardtii CCAP 11/32A glutamate + NAD+
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
 Chlamydomonas reinhardtii L-glutamate + oxidized ferredoxin
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
 Chlamydomonas reinhardtii CCAP 11/32A L-glutamate + oxidized ferredoxin
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced methylviologen artificial elctron donor Chlamydomonas reinhardtii L-glutamate + oxidized methylviologen
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced methylviologen artificial elctron donor Chlamydomonas reinhardtii CCAP 11/32A L-glutamate + oxidized methylviologen
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.14 30
-
 assay at Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.14 additional information
-
 cold-labile enzyme Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.14 7.5
-
-
 Chlamydomonas reinhardtii
1.4.7.1 7.5
-
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.14 additional information no activity with NADPH Chlamydomonas reinhardtii
1.4.1.14 NADH specific for NADH as reductant Chlamydomonas reinhardtii