EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.22 | L65A | the mutant strain lys65a produces an enzyme with greater temperature lability in vitro, a higher temperature-independent Km for Asn, and a lower temperature-dependent catalytic capacity than the enzyme from the wild-type strain | Cricetulus griseus |
EC Number | General Stability | Organism |
---|---|---|
6.1.1.22 | ATP and Mg2+ stabilize against thermal inactivation | Cricetulus griseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.22 | 0.00006 | - |
tRNAAsn | wild-type and mutant enzyme | Cricetulus griseus | |
6.1.1.22 | 0.03 | - |
Asn | wild-type | Cricetulus griseus | |
6.1.1.22 | 0.1 | - |
Asn | mutant lys65a | Cricetulus griseus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.22 | 152000 | - |
wild-type and mutant enzyme, sucrose density gradient centrifugation | Cricetulus griseus |
6.1.1.22 | 155000 | - |
gel filtration | Cricetulus griseus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.22 | Cricetulus griseus | - |
wild-type and temperature-sensitive mutant strain lys65a | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.1.1.22 | ovary | - |
Cricetulus griseus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.22 | ATP + L-asparagine + tRNAAsn | - |
Cricetulus griseus | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.22 | 37 | - |
wild-type | Cricetulus griseus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.22 | additional information | - |
ATP and Mg2+ stabilize against thermal inactivation | Cricetulus griseus |
6.1.1.22 | 37 | - |
wild-type enzyme is stable, mutant enzyme is irreversibly inactivated, half-life: 4.8 min | Cricetulus griseus |