Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rinaldi, A.; Floris, G.; Finazzi-Agro, A.
    Purification and properties of diamine oxidase from Euphorbia latex (1982), Eur. J. Biochem., 127, 417-422.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.3.22 purified enzyme does not withstand lyophilization Euphorbia characias

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.22 Arcaine sulfate
-
Euphorbia characias
1.4.3.22 cyanide
-
Euphorbia characias
1.4.3.22 diethyldithiocarbamate
-
Euphorbia characias
1.4.3.22 Diethylthiocarbamate
-
Euphorbia characias
1.4.3.22 histamine
-
Euphorbia characias
1.4.3.22 isoniazid
-
Euphorbia characias
1.4.3.22 NaCN
-
Euphorbia characias
1.4.3.22 NaN3
-
Euphorbia characias
1.4.3.22 phenylhydrazine
-
Euphorbia characias
1.4.3.22 Semicarbazide
-
Euphorbia characias

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 0.2
-
putrescine
-
Euphorbia characias
1.4.3.22 0.2
-
1,4-diaminobutane
-
Euphorbia characias
1.4.3.22 0.47
-
cadaverine
-
Euphorbia characias
1.4.3.22 0.47
-
1,5-Diaminopentane
-
Euphorbia characias

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.22 copper enzyme contains 2 Cu2+ per dimer Euphorbia characias
1.4.3.22 Manganese no manganese found Euphorbia characias

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 72000
-
2 * 72000, SDS-PAGE Euphorbia characias
1.4.3.22 140000
-
gel filtration Euphorbia characias

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Euphorbia characias
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.22 glycoprotein 12% neutral sugar Euphorbia characias

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22
-
Euphorbia characias

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.22 27
-
-
Euphorbia characias

Storage Stability

EC Number Storage Stability Organism
1.4.3.22 -20°C, in water or potassium phosphate buffer, indefinitely stable Euphorbia characias
1.4.3.22 4°C, 50% loss of activity after 15 days Euphorbia characias

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 1,10-diaminodecane + H2O + O2
-
Euphorbia characias 10-aminodecanal + NH3 + H2O2
-
?
1.4.3.22 1,4-diaminobutane + H2O + O2
-
Euphorbia characias 4-aminobutanal + NH3 + H2O2
-
?
1.4.3.22 1,5-diaminopentane + H2O + O2
-
Euphorbia characias 5-aminopentanal + NH3 + H2O2
-
?
1.4.3.22 1,6-diaminohexane + H2O + O2
-
Euphorbia characias 6-aminohexanal + NH3 + H2O2
-
?
1.4.3.22 1,7-diaminoheptane + H2O + O2
-
Euphorbia characias 7-aminoheptanal + NH3 + H2O2
-
?
1.4.3.22 RCH2NH2 + H2O + O2 no activity with spermidine Euphorbia characias RCHO + NH3 + H2O2
-
?
1.4.3.22 RCH2NH2 + H2O + O2 no activity with benzylamine Euphorbia characias RCHO + NH3 + H2O2
-
?
1.4.3.22 RCH2NH2 + H2O + O2 no activity with p-dimethylaminomethylbenzylamine Euphorbia characias RCHO + NH3 + H2O2
-
?
1.4.3.22 RCH2NH2 + H2O + O2 no activity with histamine Euphorbia characias RCHO + NH3 + H2O2
-
?
1.4.3.22 RCH2NH2 + H2O + O2 only aliphatic diamines from C4 to C10 and cystamine are oxidized, enzymatic activity decreases sharply with increasing chain length of diamines Euphorbia characias RCHO + NH3 + H2O2
-
?
1.4.3.22 spermine + H2O + O2 no reaction Euphorbia characias ?
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 dimer 2 * 72000, SDS-PAGE Euphorbia characias

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.22 7.1
-
phosphate buffer Euphorbia characias
1.4.3.22 7.6
-
in Tris buffer Euphorbia characias

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.22 additional information contains 2 carbonyl-like groups per dimer Euphorbia characias

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.22 0.00025
-
phenylhydrazine
-
Euphorbia characias
1.4.3.22 0.004
-
Arcaine sulfate
-
Euphorbia characias
1.4.3.22 0.05
-
Semicarbazide
-
Euphorbia characias
1.4.3.22 0.12
-
NaCN
-
Euphorbia characias
1.4.3.22 0.43
-
Diethylthiocarbamate
-
Euphorbia characias
1.4.3.22 6
-
histamine
-
Euphorbia characias
1.4.3.22 6
-
isoniazid
-
Euphorbia characias
1.4.3.22 54
-
NaN3
-
Euphorbia characias