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Literature summary extracted from

  • Delhaize, E.; Webb, J.
    Purification and characterization of diamine oxidase from clover leaves (1987), Phytochemistry, 26, 641-643.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 0.019
-
 cadaverine
-
 Trifolium subterraneum
1.4.3.22 0.038
-
 putrescine
-
 Trifolium subterraneum
1.4.3.22 0.074
-
 spermidine
-
 Trifolium subterraneum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.22 copper 0.063% i.e. 1.5 copper atoms per dimer Trifolium subterraneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 80000
-
 2 * 80000, gel electrophoresis under denaturing conditions Trifolium subterraneum
1.4.3.22 150000
-
 gel filtration Trifolium subterraneum

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Trifolium subterraneum
-
 clover
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22 3 isoenzymes Trifolium subterraneum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.22 leaf young folded leaves of clover Trifolium subterraneum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.22 66
-
-
 Trifolium subterraneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 cadaverine + H2O + O2
-
 Trifolium subterraneum 5-aminopentanal + NH3 + H2O2
-
 r
1.4.3.22 putrescine + H2O + O2
-
 Trifolium subterraneum 4-aminobutanal + NH3 + H2O2
-
 r
1.4.3.22 RCH2NH2 + H2O + O2
-
 Trifolium subterraneum RCHO + NH3 + H2O2
-
 ?
1.4.3.22 spermidine + H2O + O2
-
 Trifolium subterraneum ?
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.3.22 dimer 2 * 80000, gel electrophoresis under denaturing conditions Trifolium subterraneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.22 7 8 putrescine Trifolium subterraneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.22 additional information presence of one carbonyl group per enzyme dimer Trifolium subterraneum