EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.5 | wild-type and mutant enzyme | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.3.5 | enzyme in complex with pyridoxal 5'-phosphate, space group C2, 2.07 A resolution | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.5 | D49A | decrease in affinity for pyridoxine 5'-phosphate | Escherichia coli |
1.4.3.5 | H199A | decrease in affinity for pyridoxine 5'-phosphate | Escherichia coli |
1.4.3.5 | H199N | little decrease in pyridoxine 5'-phosphate turnover | Escherichia coli |
1.4.3.5 | R14E | decrease in affinity for pyridoxine 5'-phosphate | Escherichia coli |
1.4.3.5 | R14M | decrease in affinity for pyridoxine 5'-phosphate | Escherichia coli |
1.4.3.5 | R197E | strong decrease in affinity for pyridoxine 5'-phosphate | Escherichia coli |
1.4.3.5 | R197M | catalyzes removal of the proS hydrogen atom from (4'R)-3H-pyridoxamine 5'-phosphate, decrease in affinity for pyridoxine 5'-phosphate | Escherichia coli |
1.4.3.5 | Y17F | decrease in affinity for pyridoxine 5'-phosphate | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.5 | 0.0003 | - |
pyridoxine 5'-phosphate | - |
Escherichia coli | |
1.4.3.5 | 0.0003 | - |
pyridoxine 5'-phosphate | H199N mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.001 | - |
pyridoxine 5'-phosphate | D49A and Y17F mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.002 | - |
pyridoxine 5'-phosphate | R14E mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.0026 | - |
pyridoxine 5'-phosphate | R14M mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.07 | - |
pyridoxine 5'-phosphate | H199A mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.09 | - |
pyridoxine 5'-phosphate | R197M mutant enzyme | Escherichia coli | |
1.4.3.5 | 2.4 | - |
pyridoxine 5'-phosphate | R197M mutant enzyme | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.5 | Escherichia coli | P0AFI7 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.4.3.5 | pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2 | enzyme is specific for removal of the pro-R hydrogen atom from the prochiral C4' carbon atom of pyridoxamine 5'-phosphate, hydride ion mechanism is suggested | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.5 | pyridoxamine 5'-phosphate + H2O + O2 | - |
Escherichia coli | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.5 | 0.008 | - |
pyridoxine 5'-phosphate | R197M mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.03 | - |
pyridoxine 5'-phosphate | H199N mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.03 | - |
pyridoxine 5'-phosphate | R197M mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.06 | - |
pyridoxine 5'-phosphate | D49A mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.13 | - |
pyridoxine 5'-phosphate | - |
Escherichia coli | |
1.4.3.5 | 0.14 | - |
pyridoxine 5'-phosphate | H199A mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.14 | - |
pyridoxine 5'-phosphate | R14M mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.16 | - |
pyridoxine 5'-phosphate | R14E mutant enzyme | Escherichia coli | |
1.4.3.5 | 0.6 | - |
pyridoxine 5'-phosphate | Y17F mutant enzyme | Escherichia coli |