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Literature summary extracted from

  • Di Salvo, M.; Yang, E.; Zhao, G.; Winkler, M.E.; Schirch, V.
    Expression, purification, and characterization of recombinant Escherichia coli pyridoxine 5'-phosphate oxidase (1998), Protein Expr. Purif., 13, 349-356.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.5
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.002
-
 pyridoxine 5'-phosphate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.5 28000
-
 2 * 28000, SDS-PAGE Escherichia coli
1.4.3.5 51000
-
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 0.293
-
 recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Escherichia coli pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.5 dimer 2 * 28000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.5 0.2
-
 pyridoxine 5'-phosphate recombinant enzyme, in phosphate and HEPES buffer at pH 7.6 Escherichia coli
1.4.3.5 0.3
-
 pyridoxine 5'-phosphate recombinant enzyme, in Tris buffer at pH 7.6 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN 2 molecules of FMN bound per homodimer Escherichia coli