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Literature summary extracted from

  • Tsuge, H.; Ozeki, K.; Sen-Maru, K.; Ohashi, K.
    Purification and properties of pyridoxamine (pyridoxine) 5'-phosphate oxidase from baker's yeast (1979), Agric. Biol. Chem., 43, 1801-1807.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.5 Cd2+ 2.85 mM, 82% inhibition of pyridoxamine 5'-phosphate oxidation, 27% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
1.4.3.5 Cu2+ 2.85 mM, 38% inhibition of pyridoxamine 5'-phosphate oxidation, 23% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
1.4.3.5 Fe2+ 2.85 mM, 44% inhibition of pyridoxamine 5'-phosphate oxidation, 81% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
1.4.3.5 Fe3+ 2.85 mM, 26% inhibition of pyridoxamine 5'-phosphate oxidation, 50% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
1.4.3.5 Hg2+ 2.85 mM, 30% inhibition of pyridoxamine 5'-phosphate oxidation, 2% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
1.4.3.5 Zn2+ 2.85 mM, 92% inhibition of pyridoxamine 5'-phosphate oxidation, 60% inhibition of pyridoxine oxidation Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.5 additional information no requirement for bivalent cations Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 0.462
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Saccharomyces cerevisiae pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.5 45
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.5 45
-
 above 45°C, loss of activity after 30 min Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 8
-
 pyridoxine 5'-phosphate Saccharomyces cerevisiae
1.4.3.5 9
-
 pyridoxamine 5'-phosphate Saccharomyces cerevisiae