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Literature summary extracted from

  • Kazarinoff, M.N.; McCormick, D.B.
    Rabbit liver pyridoxamine (pyridoxine) 5-phosphate oxidase. Purification and properties (1975), J. Biol. Chem., 250, 3436-3442.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.3.5 repeated thawing and refreezing: denaturation Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.0059
-
 5'-methylpyridoxine-phosphate
-
 Oryctolagus cuniculus
1.4.3.5 0.0059
-
 5'-homopyridoxine-phosphate
-
 Oryctolagus cuniculus
1.4.3.5 0.01
-
 pyridoxamine 5'-phosphate
-
 Oryctolagus cuniculus
1.4.3.5 0.021
-
 pyridoxal 5'-phosphate oxime
-
 Oryctolagus cuniculus
1.4.3.5 0.021
-
 5'-phospho-pyridoxaloxime
-
 Oryctolagus cuniculus
1.4.3.5 0.025
-
 5'-phospho-pyridoxal-O-carboxymethyloxime
-
 Oryctolagus cuniculus
1.4.3.5 0.025
-
 pyridoxal 5'-phosphate-carboxymethyloxime ester
-
 Oryctolagus cuniculus
1.4.3.5 0.03
-
 5'-phospho-pyridoxyl-5-aminovalerate
-
 Oryctolagus cuniculus
1.4.3.5 0.03
-
 5'-phospho-pyridoxyl-4-aminobutyrate
-
 Oryctolagus cuniculus
1.4.3.5 0.031
-
 5'-methylpyridoxine-phosphate
-
 Oryctolagus cuniculus
1.4.3.5 0.031
-
 N-(5'-phospho-4'-pyridoxyl)-L-tyrosine
-
 Oryctolagus cuniculus
1.4.3.5 0.033
-
 N-(5'-phospho-4'-pyridoxyl)-benzylamine
-
 Oryctolagus cuniculus
1.4.3.5 0.068
-
 N-(5'-phospho-4'-pyridoxyl)-glycine
-
 Oryctolagus cuniculus
1.4.3.5 0.075
-
 N-(5'-phospho-4'-pyridoxyl)-L-leucine
-
 Oryctolagus cuniculus
1.4.3.5 0.091
-
 N-(5'-phospho-4'-pyridoxyl)-L-alpha-aminobutyrate
-
 Oryctolagus cuniculus
1.4.3.5 0.095
-
 N-(5'-phospho-4'-pyridoxyl)-L-phenylalanine
-
 Oryctolagus cuniculus
1.4.3.5 0.11
-
 N-(5'-phospho-4'-pyridoxyl)-beta-L-alanine
-
 Oryctolagus cuniculus
1.4.3.5 0.12
-
 N-(5'-phospho-4'-pyridoxyl)-L-tryptophan
-
 Oryctolagus cuniculus
1.4.3.5 0.125
-
 N-(5'-phospho-4'-pyridoxyl)-D-leucine
-
 Oryctolagus cuniculus
1.4.3.5 0.13
-
 N-(5'-phospho-4'-pyridoxyl)-L-serine
-
 Oryctolagus cuniculus
1.4.3.5 0.2
-
 alpha-N-(5'-phospho-4'-pyridoxyl)-L-lysine
-
 Oryctolagus cuniculus
1.4.3.5 0.22
-
 N-(5'-phospho-4'-pyridoxyl)-L-alanine
-
 Oryctolagus cuniculus
1.4.3.5 0.29
-
 N-(5'-phospho-4'-pyridoxyl)-D-alpha-aminobutyrate
-
 Oryctolagus cuniculus
1.4.3.5 0.4
-
 N10-(5'-phospho-4'-pyridoxyl)-1,10-diaminodecane
-
 Oryctolagus cuniculus
1.4.3.5 0.53
-
 alpha-N-(5'-phospho-4'-pyridoxyl)-ornithine
-
 Oryctolagus cuniculus
1.4.3.5 0.77
-
 N-(5'-phospho-4'-pyridoxyl)-D-alanine
-
 Oryctolagus cuniculus
1.4.3.5 1.6
-
 N-(5'-phospho-4'-pyridoxyl)-D-tyrosine
-
 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.5 27000
-
 2 * 27000, SDS-PAGE Oryctolagus cuniculus
1.4.3.5 55000
-
 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.5 liver
-
 Oryctolagus cuniculus
-

Storage Stability

EC Number Storage Stability Organism
1.4.3.5 -20°C, 18 months, over 80% of activity remains, apo- and holoenzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 5'-homopyridoxine-phosphate + H2O + O2
-
 Oryctolagus cuniculus homopyridoxal 5'-phosphate + H2O2
-
 ?
1.4.3.5 5'-methylpyridoxine-phosphate + H2O + O2
-
 Oryctolagus cuniculus ?
-
 r
1.4.3.5 5'-phospho-pyridoxal-O-carboxymethyloxime + H2O + O2
-
 Oryctolagus cuniculus O-carboxymethylpyridoxal 5'-phosphate + hydroxylamine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxaloxime + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + hydroxylamine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-4-aminobutyrate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + 4-aminobutyrate + H2O2
-
 r
1.4.3.5 5'-phospho-pyridoxyl-5-aminovalerate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + 5-aminovalerate + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-glycine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + glycine + H2O2
-
 ?
1.4.3.5 alpha-N-(5'-phospho-4'-pyridoxyl)-L-lysine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-lysine + H2O2
-
 r
1.4.3.5 alpha-N-(5'-phospho-4'-pyridoxyl)-ornithine + O2 + H2O
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + alpha-ornithine + H2O2
-
 ?
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-benzylamine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + benzylamine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-beta-L-alanine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-alanine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-D-alanine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + D-alanine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-D-alpha-aminobutyrate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + D-alpha-aminobutyrate + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-D-leucine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + D-leucine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-D-tyrosine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + D-tyrosine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-glycine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + glycine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-L-alanine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-alanine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-L-alpha-aminobutyrate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-alpha-aminobutyrate + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-L-leucine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-leucine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-L-phenylalanine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-phenylalanine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-L-serine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-serine + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-L-tryptophan + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-tryptophan + H2O2
-
 r
1.4.3.5 N-(5'-phospho-4'-pyridoxyl)-L-tyrosine + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-tyrosine + H2O2
-
 r
1.4.3.5 N10-(5'-phosphopyridoxyl)-1,10-diaminodecane + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + 1,10-diaminodecane + H2O2
-
 ?
1.4.3.5 pyridoxal 5'-phosphate oxime + NH3 + H2O2
-
 Oryctolagus cuniculus ?
-
 r
1.4.3.5 pyridoxal 5'-phosphate-carboxymethyloxime ester + NH3 + H2O2
-
 Oryctolagus cuniculus ?
-
 r
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2 no oxidation of the unphosphorylated analogs of pyridoxamine phosphate, pyridoxine phosphate and N-(phosphopyridoxyl)amines Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.5 dimer 2 * 27000, SDS-PAGE Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FAD inactive Oryctolagus cuniculus
1.4.3.5 FMN flavoprotein Oryctolagus cuniculus