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Literature summary extracted from

  • Wada, H.; Snell, E.E.
    The enzymatic oxidation of pyridoxine and pyridoxamine phosphates (1961), J. Biol. Chem., 236, 2089-2095.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.5 2-aminobutanoate activation Oryctolagus cuniculus
1.4.3.5 alanine activation Oryctolagus cuniculus
1.4.3.5 cysteine activation Oryctolagus cuniculus
1.4.3.5 Tris activation Oryctolagus cuniculus
1.4.3.5 valine activation Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.5 4-chloromercuribenzoate 0.1 mM, 90-95% inhibition Oryctolagus cuniculus
1.4.3.5 4-deoxypyridoxine 5'-phosphate 0.1 mM, 42% inhibition Oryctolagus cuniculus
1.4.3.5 4-Pyridoxic acid phosphate 0.1 mM, 33.5% inhibition Oryctolagus cuniculus
1.4.3.5 Cu2+ 1 mM, 90-95% inhibition Oryctolagus cuniculus
1.4.3.5 Hg2+ 1 mM, 90-95% inhibition Oryctolagus cuniculus
1.4.3.5 pyridoxal 5'-phosphate
-
 Oryctolagus cuniculus
1.4.3.5 pyridoxaloxime 5'-phosphate 0.2 mM, 80% inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.000031
-
 FMN
-
 Oryctolagus cuniculus
1.4.3.5 0.0031
-
 pyridoxine 5'-phosphate
-
 Oryctolagus cuniculus
1.4.3.5 0.14
-
 pyridoxamine 5'-phosphate
-
 Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 partial Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.5 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 0.432
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 9 10 pyridoxine 5'-phosphate, pyridoxamine 5'-phosphate Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FAD about 0.1% as active as FMN Oryctolagus cuniculus
1.4.3.5 FMN flavoprotein Oryctolagus cuniculus