Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Langham, L.; Garber, B.M.; Roe, D.A.; Kazarinoff, M.N.
    A radiometric assay of pyridoxamine (pyridoxine) 5-phosphate oxidase (1982), Anal. Biochem., 125, 329-334.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.5 analysis pyridoxamine phosphate oxidase assay applicable for measuring activity in erythrocytes Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.00065
-
 N-Phosphopyridoxyl tryptamine
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.5 brain
-
 Rattus norvegicus
-
1.4.3.5 erythrocyte
-
 Rattus norvegicus
-
1.4.3.5 heart
-
 Rattus norvegicus
-
1.4.3.5 intestine
-
 Rattus norvegicus
-
1.4.3.5 kidney
-
 Rattus norvegicus
-
1.4.3.5 liver
-
 Rattus norvegicus
-
1.4.3.5 lung
-
 Rattus norvegicus
-
1.4.3.5 muscle
-
 Rattus norvegicus
-
1.4.3.5 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 additional information
-
 development of a radiometric assay method, enzyme activity in erythrocytes and other tissues, overview Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 N-(5'-phosphopyridoxyl)-tryptamine + FMN + H2O radio-labeled substrate N-(5'-phosphopyridoxyl)-[3H]tryptamine Rattus norvegicus ?
-
 ?
1.4.3.5 N-phosphopyridoxyl tryptamine + H2O + O2
-
 Rattus norvegicus pyridoxal 5'-phosphate + tryptamine + H2O2
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Rattus norvegicus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 pyridoxamine (pyridoxine) 5'-phosphate oxidase
-
 Rattus norvegicus
1.4.3.5 pyridoxamine (pyridoxine) 5’-phosphate:O2 oxidoreductase (deaminating)
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.5 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 8
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN
-
 Rattus norvegicus