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Literature summary extracted from

  • Tsuge, H.; Okada, T.
    Immobilization of yeast pyridoxaminephosphate oxidase to halogenacetyl polysaccharides (1984), Biotechnol. Bioeng., 26, 412-418.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.5 synthesis continuous production of pyridoxal 5'-phosphate with enzyme immobilized to iodo- and bromoacetyl polysaccharides Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
1.4.3.5 immobilized enzyme is more stable than purified free enzyme against heat and pH change Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
Saccharomyces cerevisiae pyridoxal 5'-phosphate + NH3 + H2O2
-
?
1.4.3.5 pyridoxine 5'-phosphate + H2O + O2
-
Saccharomyces cerevisiae pyridoxal 5'-phosphate + H2O2
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.5 36
-
immobilized enzyme Saccharomyces cerevisiae
1.4.3.5 44
-
soluble enzyme Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.5 25 50 immobilized enzyme at 25°C: about 60% of maximal activity, soluble enzyme approx. 40%, immobilized enzyme at 50°C: about 30% of maximal activity, soluble enzyme approx. 20% Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.5 5
-
pH 7.0, immobilized enzyme stable for 25 days, free enzyme: rapid loss of activity Saccharomyces cerevisiae
1.4.3.5 24
-
pH 7.0, immobilized enzyme: 50% loss of activity after 10 days, free enzyme: 50% loss of activity after 2-3 days Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.5 0.103
-
pyridoxamine 5'-phosphate
-
Saccharomyces cerevisiae
1.4.3.5 0.7
-
pyridoxine 5'-phosphate
-
Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 8
-
pyridoxine 5'-phosphate, soluble and immobilized enzyme Saccharomyces cerevisiae
1.4.3.5 9
-
pyridoxamine 5'-phosphate, soluble enzyme Saccharomyces cerevisiae
1.4.3.5 9.5
-
immobilized enzyme, pyridoxamine 5'-phosphate Saccharomyces cerevisiae