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Literature summary extracted from

  • Miyata, H.; Iwamoto, A.; Kojima, Y.; Furuhashi, K.; Tsuge, H.
    Effects of culture medium on pyridoxine (pyridoxamine) phosphate oxidase activity in wheat callus (1988), Agric. Biol. Chem., 52, 343-348.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.5 ammonium in culture medium, activates PMP oxidase activity in callus culture by about 30%, no activation of PNP oxidase Triticum aestivum
1.4.3.5 potassium nitrate in culture medium, activates PNP oxidase activity 2fold in callus culture, no activation of PMP oxidase Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ Triticum aestivum
-
 pyridoxal 5'-phosphate + FMNH2 + NH3
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + FMN Triticum aestivum
-
 pyridoxal 5'-phosphate + FMNH2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Triticum aestivum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.5 callus culture cultured from immature embryos, effects of culture medium on enzyme activity in callus cultures Triticum aestivum
-
1.4.3.5 cell suspension culture
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 0.000065
-
 activity in suspension of cultered cells, substrate pyridoxamine 5'-phosphate Triticum aestivum
1.4.3.5 0.000065
-
 PMP oxidase activity in cell suspension culture Triticum aestivum
1.4.3.5 0.00007
-
 PMP oxidase activity in callus culture Triticum aestivum
1.4.3.5 0.000072
-
 activity in callus, substrate pyridoxamine 5'-phosphate Triticum aestivum
1.4.3.5 0.000097
-
 activity in cell suspension cultures, substrate pyridoxine 5'-phosphate Triticum aestivum
1.4.3.5 0.0001
-
 activity in callus, substrate pyridoxine 5'-phosphate Triticum aestivum
1.4.3.5 0.0001
-
 PNP oxidase activity in callus culture and in cell suspension culture Triticum aestivum
1.4.3.5 0.00012
-
 activity in seedlings, substrate pyridoxamine 5'-phosphate Triticum aestivum
1.4.3.5 0.00012
-
 PMP oxidase activity in seedlings Triticum aestivum
1.4.3.5 0.0005
-
 activity in seedlings, substrate pyridoxine 5'-phosphate Triticum aestivum
1.4.3.5 0.0005
-
 PNP oxidase activity in seedlings Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + FMN + H2O + 2 H+
-
 Triticum aestivum pyridoxal 5'-phosphate + FMNH2 + NH3
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Triticum aestivum pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + FMN
-
 Triticum aestivum pyridoxal 5'-phosphate + FMNH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 PMP oxidase
-
 Triticum aestivum
1.4.3.5 PNP oxidase
-
 Triticum aestivum
1.4.3.5 pyridoxamine phosphate oxidase
-
 Triticum aestivum
1.4.3.5 pyridoxine (pyridoxamine) phosphate oxidase
-
 Triticum aestivum
1.4.3.5 pyridoxine phosphate oxidase
-
 Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.5 37
-
 assay at Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 8
-
 assay at Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN
-
 Triticum aestivum